Co-Authors:
Marco, S., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Cohen, S., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Harpaz, I., Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Birk, Y., Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Abstract:
An anti-viral factor (PARFa), related to the 'periodic acquisition' phenomenon of the tomato yellow leaf curl virus (TYLCV) by the tobacco whitefly, was investigated by ion-exchange chromatography and some of its properties were determined. Separating crude PARFa on a DEAE-cellulose column revealed the presence of two different active fractions. Both fractions were found to occur periodically in the vector's body in concurrence with TYLCV inoculability. PARFa was sensitive to temperatures over 35°C, resistant to pH changes in the range of 2·2 to 9·0 and to RNase, but lost its activity after exposure to pronase. © 1975.