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Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated
Year:
2007
Source of publication :
Plant Cell
Authors :
Arazi, Tzahi
;
.
Azoulay, Tamar
;
.
Eyal, Yoram
;
.
Gal-On, Amit
;
.
Gidoni, David
;
.
Mett, Anahit
;
.
Volume :
19
Co-Authors:
Harpaz-Saad, S., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Azoulay, T., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Arazi, T., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Ben-Yaakov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mett, A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Shiboleth, Y.M., Institute of Plant Protection, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Hörtensteiner, S., Institute of Plant Sciences, University of Bern, CH-3013 Bern, Switzerland
Gidoni, D., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Gal-On, A., Institute of Plant Protection, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Goldschmidt, E.E., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Facilitators :
From page:
1007
To page:
1022
(
Total pages:
16
)
Abstract:
Chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. To study the role and regulation of chlorophyllase (Chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (Citrus sinensis) Chlase in two heterologous plant systems: (1) squash (Cucurbita pepo) plants using a viral vector expression system; and (2) transiently transformed tobacco (Nicotiana tabacum) protoplasts. Expression of full-length citrus Chlase resulted in limited chlorophyll breakdown in protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids (ChlaseΔN), which corresponds to the mature protein, led to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves. ChlaseΔN-expressing squash leaves displayed a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurred, which was demonstrated to follow the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product. Full-length and mature citrus Chlase versions were localized to the chloroplast membrane fraction in expressing tobacco protoplasts, where processing of the N-terminal 21 amino acids appears to occur. Results obtained in both plant systems suggest that Chlase functions as a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation. © 2007 American Society of Plant Biologists.
Note:
Related Files :
chlorophyll
Cucurbita
Cucurbita pepo
gene expression
Genetics
phenotype
photosynthesis
Plants
Show More
Related Content
More details
DOI :
10.1105/tpc.107.050633
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20649
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:38
You may also be interested in
Scientific Publication
Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated
19
Harpaz-Saad, S., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Azoulay, T., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Arazi, T., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Ben-Yaakov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mett, A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Shiboleth, Y.M., Institute of Plant Protection, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Hörtensteiner, S., Institute of Plant Sciences, University of Bern, CH-3013 Bern, Switzerland
Gidoni, D., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Gal-On, A., Institute of Plant Protection, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Goldschmidt, E.E., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated
Chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. To study the role and regulation of chlorophyllase (Chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (Citrus sinensis) Chlase in two heterologous plant systems: (1) squash (Cucurbita pepo) plants using a viral vector expression system; and (2) transiently transformed tobacco (Nicotiana tabacum) protoplasts. Expression of full-length citrus Chlase resulted in limited chlorophyll breakdown in protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids (ChlaseΔN), which corresponds to the mature protein, led to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves. ChlaseΔN-expressing squash leaves displayed a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurred, which was demonstrated to follow the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product. Full-length and mature citrus Chlase versions were localized to the chloroplast membrane fraction in expressing tobacco protoplasts, where processing of the N-terminal 21 amino acids appears to occur. Results obtained in both plant systems suggest that Chlase functions as a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation. © 2007 American Society of Plant Biologists.
Scientific Publication
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