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Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis
Year:
2002
Source of publication :
Molecular and Cellular Biology
Authors :
Faerman, Alexander
;
.
Moshel, Yana
;
.
Volume :
22
Co-Authors:
Shoshani, T., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Faerman, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Mett, I., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Zelin, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Tenne, T., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Gorodin, S., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Moshel, Y., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Elbaz, S., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Budanov, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Chajut, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Kalinski, H., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Kamer, I., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Rozen, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Mor, O., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Keshet, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Leshkowitz, D., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Einat, P., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Skaliter, R., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Feinstein, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Facilitators :
From page:
2283
To page:
2293
(
Total pages:
11
)
Abstract:
Hypoxia is an important factor that elicits numerous physiological and pathological responses. One of the major gene expression programs triggered by hypoxia is mediated through hypoxia-responsive transcription factor hypoxia-inducible factor 1 (HIF-1). Here, we report the identification and cloning of a novel HIF-1-responsive gene, designated RTP801. Its strong up-regulation by hypoxia was detected both in vitro and in vivo in an animal model of ischemic stroke. When induced from a tetracycline-repressible promoter, RTP801 protected MCF7 and PC12 cells from hypoxia in glucose-free medium and from H2O2-triggered apoptosis via a dramatic reduction in the generation of reactive oxygen species. However, expression of RTP801 appeared toxic for nondividing neuron-like PC12 cells and increased their sensitivity to ischemic injury and oxidative stress. Liposomal delivery of RTP801 cDNA to mouse lungs also resulted in massive cell death. Thus, the biological effect of RTP801 overexpression depends on the cell context and may be either protecting or detrimental for cells under conditions of oxidative or ischemic stresses. Altogether, the data suggest a complex type of involvement of RTP801 in the pathogenesis of ischemic diseases.
Note:
Related Files :
Animals
apoptosis
Gene
gene expression
hypoxia
mice
Nuclear Proteins
PC12 Cells
reactive oxygen species
Show More
Related Content
More details
DOI :
10.1128/MCB.22.7.2283-2293.2002
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20995
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:40
Scientific Publication
Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis
22
Shoshani, T., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Faerman, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Mett, I., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Zelin, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Tenne, T., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Gorodin, S., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Moshel, Y., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Elbaz, S., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Budanov, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Chajut, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Kalinski, H., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Kamer, I., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Rozen, A., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Mor, O., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Keshet, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Leshkowitz, D., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Einat, P., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Skaliter, R., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Feinstein, E., Quark Biotech Inc./QBI Entpr., Ltd., Weizmann Science Park, POB 4071, Ness Ziona 70400, Israel
Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis
Hypoxia is an important factor that elicits numerous physiological and pathological responses. One of the major gene expression programs triggered by hypoxia is mediated through hypoxia-responsive transcription factor hypoxia-inducible factor 1 (HIF-1). Here, we report the identification and cloning of a novel HIF-1-responsive gene, designated RTP801. Its strong up-regulation by hypoxia was detected both in vitro and in vivo in an animal model of ischemic stroke. When induced from a tetracycline-repressible promoter, RTP801 protected MCF7 and PC12 cells from hypoxia in glucose-free medium and from H2O2-triggered apoptosis via a dramatic reduction in the generation of reactive oxygen species. However, expression of RTP801 appeared toxic for nondividing neuron-like PC12 cells and increased their sensitivity to ischemic injury and oxidative stress. Liposomal delivery of RTP801 cDNA to mouse lungs also resulted in massive cell death. Thus, the biological effect of RTP801 overexpression depends on the cell context and may be either protecting or detrimental for cells under conditions of oxidative or ischemic stresses. Altogether, the data suggest a complex type of involvement of RTP801 in the pathogenesis of ischemic diseases.
Scientific Publication
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