נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones
Year:
2005
Source of publication :
Plant Cell
Authors :
Fridman, Eyal
;
.
Volume :
17
Co-Authors:
Fridman, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Wang, J., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States, Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada
Iijima, Y., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Froehlich, J.E., Michigan State University, Department of Energy, Plant Research Laboratory, East Lansing, MI 48824, United States
Gang, D.R., Department of Plant Biology, University of Arizona, Tuscon, AZ 85721-0036, United States
Ohlrogge, J., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Pichersky, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Facilitators :
From page:
1252
To page:
1267
(
Total pages:
16
)
Abstract:
Medium-length methylketones (C7-C15) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato Lycopersicon hirsutum f glabratum (accession PI126449). A comparison of gland EST databases from accession PI126449 and a second L. hirsutum accession, LA1777, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated Methylketone Synthase 1 (MKS1), was expressed in Escherichia coli and the purified protein used to catalyze in vitro reactions in which C 12, C14, and C16 β-ketoacyl-acyl-carrier- proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C11, C13, and C15 methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of MKS1 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs. © 2005 American Society of Plant Biologists.
Note:
Related Files :
Biochemistry
biosynthesis
enzymes
fatty acids
Genetics
genomics
molecular genetics
Plants
Show More
Related Content
More details
DOI :
10.1105/tpc.104.029736
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21032
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
You may also be interested in
Scientific Publication
Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones
17
Fridman, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Wang, J., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States, Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada
Iijima, Y., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Froehlich, J.E., Michigan State University, Department of Energy, Plant Research Laboratory, East Lansing, MI 48824, United States
Gang, D.R., Department of Plant Biology, University of Arizona, Tuscon, AZ 85721-0036, United States
Ohlrogge, J., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Pichersky, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones
Medium-length methylketones (C7-C15) are highly effective in protecting plants from numerous pests. We used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato Lycopersicon hirsutum f glabratum (accession PI126449). A comparison of gland EST databases from accession PI126449 and a second L. hirsutum accession, LA1777, whose glands do not contain methylketones, showed that the expression of genes for fatty acid biosynthesis is elevated in PI126449 glands, suggesting de novo biosynthesis of methylketones. A cDNA abundant in the PI126449 gland EST database but rare in the LA1777 database was similar in sequence to plant esterases. This cDNA, designated Methylketone Synthase 1 (MKS1), was expressed in Escherichia coli and the purified protein used to catalyze in vitro reactions in which C 12, C14, and C16 β-ketoacyl-acyl-carrier- proteins (intermediates in fatty acid biosynthesis) were hydrolyzed and decarboxylated to give C11, C13, and C15 methylketones, respectively. Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it was targeted to the stroma of plastids, where fatty acid biosynthesis occurs. Levels of MKS1 transcript, protein, and enzymatic activity were correlated with levels of methylketones and gland density in a variety of tomato accessions and in different plant organs. © 2005 American Society of Plant Biologists.
Scientific Publication
You may also be interested in