Co-Authors:
Wilchek, M., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, S., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, Y., The Nematology, Dept. ARO, The Volcani Center, Bet Dagan, Israel
Abstract:
Rhodamine and fluoresceine containing hydrazides were synthesized and used for fluorescent labeling of glycoconjugates on cell surface or in solution. The procedure involves the oxidation of the glycoconjugates with sodium metaperiodate or galactose oxidase to form an aldehyde group which reacts with the respective hydrazides. The method was applied for the modification of cell surface sialic acid and galactose residues on thymocytes and nematodes as well as for the labeling of glycoproteins and gangliosides in solution. The many possible application of these highly fluorescent compounds in the study of cell surface events is considered. © 1980.
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