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Fluorescent reagents for the labeling of glycoconjugates in solution and on cell surfaces
Year:
1980
Authors :
Spiegel, Yitzhak
;
.
Volume :
92
Co-Authors:
Wilchek, M., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, S., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, Y., The Nematology, Dept. ARO, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
1215
To page:
1222
(
Total pages:
8
)
Abstract:
Rhodamine and fluoresceine containing hydrazides were synthesized and used for fluorescent labeling of glycoconjugates on cell surface or in solution. The procedure involves the oxidation of the glycoconjugates with sodium metaperiodate or galactose oxidase to form an aldehyde group which reacts with the respective hydrazides. The method was applied for the modification of cell surface sialic acid and galactose residues on thymocytes and nematodes as well as for the labeling of glycoproteins and gangliosides in solution. The many possible application of these highly fluorescent compounds in the study of cell surface events is considered. © 1980.
Note:
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More details
DOI :
10.1016/0006-291X(80)90416-7
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21038
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Fluorescent reagents for the labeling of glycoconjugates in solution and on cell surfaces
92
Wilchek, M., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, S., Dept. of Biophysics, Weizmann Institute of Science, Rehovot, Israel
Spiegel, Y., The Nematology, Dept. ARO, The Volcani Center, Bet Dagan, Israel
Fluorescent reagents for the labeling of glycoconjugates in solution and on cell surfaces
Rhodamine and fluoresceine containing hydrazides were synthesized and used for fluorescent labeling of glycoconjugates on cell surface or in solution. The procedure involves the oxidation of the glycoconjugates with sodium metaperiodate or galactose oxidase to form an aldehyde group which reacts with the respective hydrazides. The method was applied for the modification of cell surface sialic acid and galactose residues on thymocytes and nematodes as well as for the labeling of glycoproteins and gangliosides in solution. The many possible application of these highly fluorescent compounds in the study of cell surface events is considered. © 1980.
Scientific Publication
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