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Techniques for the isolation and identification of steroids in insects and algae
Year:
1980
Source of publication :
Lipids
Authors :
Svoboda, James
;
.
Volume :
15
Co-Authors:
Thompson, M.J., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Patterson, G.W., Department of Botany, University of Maryland, College Park, 20742, MD, United States
Dutky, S.R., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Kaplanis, J.N., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Facilitators :
From page:
719
To page:
733
(
Total pages:
15
)
Abstract:
Analytical techniques, methods and instrumentation employed for the extraction, isolation separation, purification and identification of steroids from algae and insects are presented. The techniques include adsorption and argentation column chromatography and counter-current distribution for separating the individual steroids, and thin layer chromatography and gas liquid chromatography for monitoring the purification process. Double bond, steric and alkyl substituent separation factors and relative retention times are reported for a large number of sterols on 4 different column systems. Their use permits the tentative structural assignment of sterols. Ultraviolet, infrared, nuclear magnetic resonance (NMR) and mass spectral analyses are discussed in light of their significance in the isolation and identification of steroids from insects and algae. Numerous examples are presented, including the use of 220 MHz NMR spectrometry, which permits the differentiation and characterization of C-24 epimetric sterols and allows for a semiquantitative estimate of the 24α- and 24β-epimers present in a mixture. © 1980 American Oil Chemists' Society.
Note:
Related Files :
alga
algae
animal experiment
Animalia
arthropod
insect
Insecta
invertebrate
steroid
Show More
Related Content
More details
DOI :
10.1007/BF02534026
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21040
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
Scientific Publication
Techniques for the isolation and identification of steroids in insects and algae
15
Thompson, M.J., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Patterson, G.W., Department of Botany, University of Maryland, College Park, 20742, MD, United States
Dutky, S.R., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Svoboda, J.A., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Kaplanis, J.N., Insect Physiology Laboratory, Agricultural Research, Science and Education Administration, Beltsville, 20705, MD, United States
Techniques for the isolation and identification of steroids in insects and algae
Analytical techniques, methods and instrumentation employed for the extraction, isolation separation, purification and identification of steroids from algae and insects are presented. The techniques include adsorption and argentation column chromatography and counter-current distribution for separating the individual steroids, and thin layer chromatography and gas liquid chromatography for monitoring the purification process. Double bond, steric and alkyl substituent separation factors and relative retention times are reported for a large number of sterols on 4 different column systems. Their use permits the tentative structural assignment of sterols. Ultraviolet, infrared, nuclear magnetic resonance (NMR) and mass spectral analyses are discussed in light of their significance in the isolation and identification of steroids from insects and algae. Numerous examples are presented, including the use of 220 MHz NMR spectrometry, which permits the differentiation and characterization of C-24 epimetric sterols and allows for a semiquantitative estimate of the 24α- and 24β-epimers present in a mixture. © 1980 American Oil Chemists' Society.
Scientific Publication
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