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Determination of Poly(ethylene glycol)-Binding to Browse Foliage, as an Assay of Tannin, by Near-Infrared Reflectance Spectroscopy
Year:
2004
Authors :
Dvash, Levana
;
.
Landau, Serge Yan
;
.
Shapiro, Fira
;
.
Silanikove, Nissim
;
.
Volume :
52
Co-Authors:
Landau, S., Institute of Field and Garden Crops, Department of Natural Resources, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Dvash, L., Institute of Field and Garden Crops, Department of Natural Resources, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Decandia, M., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Cabiddu, A., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Shapiro, F., Dept. of Nutr./Physiol. of Cattle, Institute of Animal Science, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Molle, G., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Silanikove, N., Dept. of Nutr./Physiol. of Cattle, Institute of Animal Science, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Facilitators :
From page:
638
To page:
642
(
Total pages:
5
)
Abstract:
Nutritionists are interested in functional assays of tannins that do not require time-consuming and expensive extraction, such as the 14C-labeled poly(ethylene glycol) (PEG)-binding (PEG-b) assay. This paper reports the application of near-infrared (NIR) spectroscopy to determine the percentage of PEG binding, in place of the 14C-labeled PEG-b assay of tannin, in Mediterranean woodland vegetation. Calibration was done with 53 samples from 14 species and was validated on 25 samples from 10 species. PEG-b ranged between 1.4 and 20.7% in the samples. The calibration obtained by using the modified partial least-squares (MPLS) method, with all wavelengths in the 1100-2500 nm range combined, and the validation were reasonably linear (R 2 = 0.96 and 0.91, respectively). The accuracies, estimated from the standard errors of cross-validation and prediction, were ±1.6 and ±17% PEG-b, respectively. The NIRS-aided procedure proposed here can serve as an accurate, inexpensive, time-saving, and environment-friendly functional assay of tannin in Mediterranean browse.
Note:
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21043
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Determination of Poly(ethylene glycol)-Binding to Browse Foliage, as an Assay of Tannin, by Near-Infrared Reflectance Spectroscopy
52
Landau, S., Institute of Field and Garden Crops, Department of Natural Resources, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Dvash, L., Institute of Field and Garden Crops, Department of Natural Resources, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Decandia, M., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Cabiddu, A., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Shapiro, F., Dept. of Nutr./Physiol. of Cattle, Institute of Animal Science, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Molle, G., Ist. Zootecnico/Caseario Sardegna, Olmedo (SS) 07040, Italy
Silanikove, N., Dept. of Nutr./Physiol. of Cattle, Institute of Animal Science, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Determination of Poly(ethylene glycol)-Binding to Browse Foliage, as an Assay of Tannin, by Near-Infrared Reflectance Spectroscopy
Nutritionists are interested in functional assays of tannins that do not require time-consuming and expensive extraction, such as the 14C-labeled poly(ethylene glycol) (PEG)-binding (PEG-b) assay. This paper reports the application of near-infrared (NIR) spectroscopy to determine the percentage of PEG binding, in place of the 14C-labeled PEG-b assay of tannin, in Mediterranean woodland vegetation. Calibration was done with 53 samples from 14 species and was validated on 25 samples from 10 species. PEG-b ranged between 1.4 and 20.7% in the samples. The calibration obtained by using the modified partial least-squares (MPLS) method, with all wavelengths in the 1100-2500 nm range combined, and the validation were reasonably linear (R 2 = 0.96 and 0.91, respectively). The accuracies, estimated from the standard errors of cross-validation and prediction, were ±1.6 and ±17% PEG-b, respectively. The NIRS-aided procedure proposed here can serve as an accurate, inexpensive, time-saving, and environment-friendly functional assay of tannin in Mediterranean browse.
Scientific Publication
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