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Properties of Prostaglandin Synthetase of Rabbit Kidney Medulla
Year:
1976
Source of publication :
European Journal of Biochemistry
Authors :
Gafni, Yedidya
;
.
Volume :
64
Co-Authors:
SCHWARTZMAN, M., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
GAFNI, Y., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
RAZ, A., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
Facilitators :
From page:
527
To page:
534
(
Total pages:
8
)
Abstract:
The formation in vitro of prostaglandins E2, D2 and F2α from arachidonic acid by rabbit kidney medulla homogenate or microsomal fraction is markedly affected by the composition of the incubation medium employed. Optimal biosynthesis is obtained in 0.1 M potassium phosphate buffer, with the optimum pH being 8.0–8.8. Under these conditions prostaglandin formation is linear up to arachidonic acid concentration of 30 μM. The initial rate of formation of prostaglandin E2+ prostaglandin D2 is 3–4 times higher than that of prostaglandin F2α. Reduced glutathione (1 mM) did not affect the biosynthesis by medulla homogenate and produced only small stimulation of the biosynthesis by microsomal powder. Hydroquinone produced a small stimulation at a low concentration of 0.005 mM, and a strong inhibition at concentrations of 0.1 mM or higher. Addition of bovine serum albumin (0.1%) reduced the microsomal biosynthesis of prostaglandins by approximately 80%. Addition of boiled homogenate or boiled 140000 ×g supernatant produced small stimulation of microsomal biosynthesis while 140000 ×g supernatant (not boiled) caused small inhibition which was not dose‐related. It appears that rabbit kidney prostaglandin‐synthetase converts arachidonic acid to prostaglandins E2 and F2α in comparable amounts, without apparent need for a cytoplasmic soluble cofactor or specific reducing agents. Copyright © 1976, Wiley Blackwell. All rights reserved
Note:
Related Files :
Animal
arachidonic acid c 14
Kidney Medulla
Male
Prostaglandins
Seminal Vesicles
sheep
Show More
Related Content
More details
DOI :
10.1111/j.1432-1033.1976.tb10332.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21047
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Properties of Prostaglandin Synthetase of Rabbit Kidney Medulla
64
SCHWARTZMAN, M., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
GAFNI, Y., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
RAZ, A., Department of Biochemistry, George S. Wise Center of Life Sciences, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel
Properties of Prostaglandin Synthetase of Rabbit Kidney Medulla
The formation in vitro of prostaglandins E2, D2 and F2α from arachidonic acid by rabbit kidney medulla homogenate or microsomal fraction is markedly affected by the composition of the incubation medium employed. Optimal biosynthesis is obtained in 0.1 M potassium phosphate buffer, with the optimum pH being 8.0–8.8. Under these conditions prostaglandin formation is linear up to arachidonic acid concentration of 30 μM. The initial rate of formation of prostaglandin E2+ prostaglandin D2 is 3–4 times higher than that of prostaglandin F2α. Reduced glutathione (1 mM) did not affect the biosynthesis by medulla homogenate and produced only small stimulation of the biosynthesis by microsomal powder. Hydroquinone produced a small stimulation at a low concentration of 0.005 mM, and a strong inhibition at concentrations of 0.1 mM or higher. Addition of bovine serum albumin (0.1%) reduced the microsomal biosynthesis of prostaglandins by approximately 80%. Addition of boiled homogenate or boiled 140000 ×g supernatant produced small stimulation of microsomal biosynthesis while 140000 ×g supernatant (not boiled) caused small inhibition which was not dose‐related. It appears that rabbit kidney prostaglandin‐synthetase converts arachidonic acid to prostaglandins E2 and F2α in comparable amounts, without apparent need for a cytoplasmic soluble cofactor or specific reducing agents. Copyright © 1976, Wiley Blackwell. All rights reserved
Scientific Publication
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