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Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanica
Year:
2011
Source of publication :
Protoplasma
Authors :
Abu-Abied, Mohamad
;
.
Altshuler, Osnat
;
.
Cohen, Avner
;
.
Moskovitz, Yoni
;
.
Perl, Avi
;
.
Sadot, Einat
;
.
Weissberg, Mira
;
.
Volume :
248
Co-Authors:
Halpert, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel, Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Abu-Abied, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Avisar, D., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Moskovitz, Y., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Altshuler, O., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Cohen, A., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Weissberg, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Riov, J., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Gottlieb, H.E., Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel
Perl, A., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Sadot, E., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
785
To page:
797
(
Total pages:
13
)
Abstract:
Plants are an infinite source of bioactive compounds. We screened the Israeli flora for compounds that interfere with the organization of the actin cytoskeleton. We found an activity in lipidic extract from Iris germanica that was able to increase HeLa cell area and adhesion and augment the formation of actin stress fibers. This effect was not observed when Ref52 fibroblasts were tested and was not the result of disruption of microtubules. Further, the increase in cell area was Rac1-dependent, and the iris extract led to slight Rac activation. Inhibitor of RhoA kinase did not interfere with the ability of the iris extract to increase HeLa cell area. The increase in HeLa cell area in the presence of iris extract was accompanied by impairment of cell migration and arrest of the cell cycle at G1 although the involvement of Rac1 in these processes is not clear. Biochemical verification of the extract based on activity-mediated fractionation and nuclear magnetic resonance analysis revealed that the active compounds belong to the group of iridals, a known group of triterpenoid. Purified iripallidal was able to increase cell area of both HeLa and SW480 cells. © 2011 Springer-Verlag.
Note:
Related Files :
Animals
metabolism
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More details
DOI :
10.1007/s00709-010-0254-1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21056
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
Scientific Publication
Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanica
248
Halpert, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel, Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Abu-Abied, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Avisar, D., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Moskovitz, Y., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Altshuler, O., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Cohen, A., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Weissberg, M., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Riov, J., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Gottlieb, H.E., Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel
Perl, A., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Sadot, E., The Institute of Plant Sciences, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanica
Plants are an infinite source of bioactive compounds. We screened the Israeli flora for compounds that interfere with the organization of the actin cytoskeleton. We found an activity in lipidic extract from Iris germanica that was able to increase HeLa cell area and adhesion and augment the formation of actin stress fibers. This effect was not observed when Ref52 fibroblasts were tested and was not the result of disruption of microtubules. Further, the increase in cell area was Rac1-dependent, and the iris extract led to slight Rac activation. Inhibitor of RhoA kinase did not interfere with the ability of the iris extract to increase HeLa cell area. The increase in HeLa cell area in the presence of iris extract was accompanied by impairment of cell migration and arrest of the cell cycle at G1 although the involvement of Rac1 in these processes is not clear. Biochemical verification of the extract based on activity-mediated fractionation and nuclear magnetic resonance analysis revealed that the active compounds belong to the group of iridals, a known group of triterpenoid. Purified iripallidal was able to increase cell area of both HeLa and SW480 cells. © 2011 Springer-Verlag.
Scientific Publication
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