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Interference of azide in assays of carbohydrates
Year:
1984
Source of publication :
Food Chemistry
Authors :
Lindner, Pinhas
;
.
Shomer, Ilan
;
.
Volume :
14
Co-Authors:
Lindner, P., Department of Food Technology, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Shomer, I., Department of Food Technology, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
141
To page:
153
(
Total pages:
13
)
Abstract:
The effect of sodium azide at concentrations of 0.02% on three carbohydrate assay methods was investigated: it reduced the sensitivity of the phenol-sulfuric acid method for aldoses by 30-35%; the m-hydroxydiphenyl and orcinol methods for galacturonic acid became insensitive. At this level of azide the sensitivity of the orcinol method for neutral sugar, but not for galacturonic acid, could be partially restored by increasing the amount of orcinol in the reaction mixture. The antimicrobial agent thimerosal did not interfere with the phenol-sulfuric acid or m-hydroxybiphenyl methods; with the orcinol method it interfered with the determination of galacturonic acid. This latter problem can be overcome by measuring the absorbance at longer wavelengths or correcting the measurements by an appropriate blank value. © 1984.
Note:
Related Files :
azide
Carbohydrate
nonhuman
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More details
DOI :
10.1016/0308-8146(84)90053-0
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21085
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Interference of azide in assays of carbohydrates
14
Lindner, P., Department of Food Technology, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Shomer, I., Department of Food Technology, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Interference of azide in assays of carbohydrates
The effect of sodium azide at concentrations of 0.02% on three carbohydrate assay methods was investigated: it reduced the sensitivity of the phenol-sulfuric acid method for aldoses by 30-35%; the m-hydroxydiphenyl and orcinol methods for galacturonic acid became insensitive. At this level of azide the sensitivity of the orcinol method for neutral sugar, but not for galacturonic acid, could be partially restored by increasing the amount of orcinol in the reaction mixture. The antimicrobial agent thimerosal did not interfere with the phenol-sulfuric acid or m-hydroxybiphenyl methods; with the orcinol method it interfered with the determination of galacturonic acid. This latter problem can be overcome by measuring the absorbance at longer wavelengths or correcting the measurements by an appropriate blank value. © 1984.
Scientific Publication
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