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Induced diploid gynogenesis and polyploidy in the ornamental (koi) carp Cyprinus carpio L. - 3. Optimization of heat-shock timing during the 2nd meiotic division and the 1st cleavage
Year:
1994
Source of publication :
Theoretical and Applied Genetics
Authors :
Ben-Dom, Naomi
;
.
Cherfas, Nina B.
;
.
Hulata, Gideon
;
.
Volume :
89
Co-Authors:
Cherfas, N.B., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Peretz, Y., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Ben-Dom, N., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Gomelsky, B., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Hulata, G., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Facilitators :
From page:
281
To page:
286
(
Total pages:
6
)
Abstract:
The results of a series of experiments conducted in our laboratory on the ornamental common carp (koi), aimed at optimizing heat-shock chromosome-set manipulation procedures, are described. The timing of heat-shock initiation was expressed in the relative unit of embryological age (τ0) in order to standardize this parameter, the absolute time for heat-shock initiation being calculated from duration of one τ0 at two different pre-treatment water temperatures. Heat shocks were applied within the periods of 0.05-0.60 τ0 and 1.20-2.20 τ0 which, respectively, cover the successive phases of the 2nd meiotic division and the 1st cleavage. The highest production of diploid gynogenetic offspring was observed when heat shocks were initiated at 0.15-0.25 τ0 and at 1.5 τ0, after insemination, corresponding to anaphase of meiosis-II, and metaphase of the 1st cleavage, respectively. Similar results were obtained irrespective of the different pre-treatment water temperatures, thus confirming the possibility of standardizing heat-shock timing by τ0. © 1994 Springer-Verlag.
Note:
Related Files :
Chromosome-set manipulations
Common carp
Heat-shock timing
Induced gynogenesis
Show More
Related Content
More details
DOI :
10.1007/BF00225155
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21087
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Induced diploid gynogenesis and polyploidy in the ornamental (koi) carp Cyprinus carpio L. - 3. Optimization of heat-shock timing during the 2nd meiotic division and the 1st cleavage
89
Cherfas, N.B., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Peretz, Y., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Ben-Dom, N., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Gomelsky, B., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Hulata, G., Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel
Induced diploid gynogenesis and polyploidy in the ornamental (koi) carp Cyprinus carpio L. - 3. Optimization of heat-shock timing during the 2nd meiotic division and the 1st cleavage
The results of a series of experiments conducted in our laboratory on the ornamental common carp (koi), aimed at optimizing heat-shock chromosome-set manipulation procedures, are described. The timing of heat-shock initiation was expressed in the relative unit of embryological age (τ0) in order to standardize this parameter, the absolute time for heat-shock initiation being calculated from duration of one τ0 at two different pre-treatment water temperatures. Heat shocks were applied within the periods of 0.05-0.60 τ0 and 1.20-2.20 τ0 which, respectively, cover the successive phases of the 2nd meiotic division and the 1st cleavage. The highest production of diploid gynogenetic offspring was observed when heat shocks were initiated at 0.15-0.25 τ0 and at 1.5 τ0, after insemination, corresponding to anaphase of meiosis-II, and metaphase of the 1st cleavage, respectively. Similar results were obtained irrespective of the different pre-treatment water temperatures, thus confirming the possibility of standardizing heat-shock timing by τ0. © 1994 Springer-Verlag.
Scientific Publication
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