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DNA fingerprint analysis of ornamental plants
Year:
1991
Source of publication :
Plant Science
Authors :
Haberfeld, Alon
;
.
Lavi, Uri
;
.
Tzuri, Galil
;
.
Volume :
76
Co-Authors:
Tzuri, G., Department of Horticulture and the Otto Warburg Center for Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Hillel, J., Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Lavi, U., Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Haberfeld, A., Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Vainstein, A., Department of Horticulture and the Otto Warburg Center for Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Facilitators :
From page:
91
To page:
97
(
Total pages:
7
)
Abstract:
Minisatellite and microsatellite DNA probes hybridize to many loci in rose, gerbera and carnation DNA, allowing cultivar identification in these ornamentals. The level of polymorphism revealed by DNA fingerprints is probe/genus dependent. Jeffrey's probe 33.6 yielded DNA fingerprints in all genera analyzed, while Jeffreys' probe 33.15 did not yield any detectable signal in gerbera. Cattle derived probe R18.1 detected a higher proportion of polymorphic DNA fingerprint bands in carnation than did any of the other probes. Hybridization of the oligonucleotide probe (GT)12 revealed that simple GT microsatellite repeats generated the hybridization pattern obtained by probe R18.1. Full-sib family analysis of DNA fingerprints prepared from 'standard' Dianthus caryophyllus progeny revealed 36 scorable parental specific bands out of the 186 observed in the parents. These bands were detected cumulatively by phage (M13), cattle (R18.1) and human derived (33.6, 33.15) probes. Only two pairs of these loci were found to be linked. No allelic pairs were detected in the analyzed progeny. The probability of two offspring from a 'standard' carnation intraspecific cross having identical DNA fingerprint patterns was calculated as 1.8 × 10-6. © 1991.
Note:
Related Files :
carnation
DNA fingerprints
Microsatellites
Minisatellites
ornamentals
Show More
Related Content
More details
DOI :
10.1016/0168-9452(91)90222-T
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21091
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
Scientific Publication
DNA fingerprint analysis of ornamental plants
76
Tzuri, G., Department of Horticulture and the Otto Warburg Center for Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Hillel, J., Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Lavi, U., Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Haberfeld, A., Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
Vainstein, A., Department of Horticulture and the Otto Warburg Center for Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
DNA fingerprint analysis of ornamental plants
Minisatellite and microsatellite DNA probes hybridize to many loci in rose, gerbera and carnation DNA, allowing cultivar identification in these ornamentals. The level of polymorphism revealed by DNA fingerprints is probe/genus dependent. Jeffrey's probe 33.6 yielded DNA fingerprints in all genera analyzed, while Jeffreys' probe 33.15 did not yield any detectable signal in gerbera. Cattle derived probe R18.1 detected a higher proportion of polymorphic DNA fingerprint bands in carnation than did any of the other probes. Hybridization of the oligonucleotide probe (GT)12 revealed that simple GT microsatellite repeats generated the hybridization pattern obtained by probe R18.1. Full-sib family analysis of DNA fingerprints prepared from 'standard' Dianthus caryophyllus progeny revealed 36 scorable parental specific bands out of the 186 observed in the parents. These bands were detected cumulatively by phage (M13), cattle (R18.1) and human derived (33.6, 33.15) probes. Only two pairs of these loci were found to be linked. No allelic pairs were detected in the analyzed progeny. The probability of two offspring from a 'standard' carnation intraspecific cross having identical DNA fingerprint patterns was calculated as 1.8 × 10-6. © 1991.
Scientific Publication
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