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Analysis of quantitative interactions between two species of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices, by real-time PCR
Year:
2006
Authors :
Alkan, Noam
;
.
Kapulnik, Yoram
;
.
Volume :
72
Co-Authors:
Alkan, N., Department of Agronomy and Natural Resources, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Gadkar, V., Microbial Ecology Program, Division of Biological Sciences, University of Montana, 32 Campus Dr. 4824, Missoula, MT 59812, United States
Yarden, Q., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76 100, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Facilitators :
From page:
4192
To page:
4199
(
Total pages:
8
)
Abstract:
Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs, known to play an important role in ecological processes. Conventional light microscopy is the most common method used to detect their presence in planta, but this method fails to discern the presence of multiple AMF species and is not quantitative. These two factors are critically important in ecological studies, where the symbiotic contribution of each isolate needs to be defined. This paper describes the use of quantitative real-time PCR (qRT-PCR) as a detection system to address this issue. We used two Glomus spp., namely, G. intraradices and G. mosseae, to show that it is possible to study the interactions between these two isolates during the cocolonization of a single root system. Three different physiological studies were set up to assess how the interactions affected the occupancy of these fungi intraradically on a temporal basis. These treatments included saline and phosphorus stress, spatial distribution in the root zone, and preference for a particular host. qRT-PCR could prove a valuable tool in the area of AMF field ecology, where such data are critically important for defining the role of each species in the community structure. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Note:
Related Files :
biodiversity
Ecology
fungi
plant
stress
Symbiosis
Templates, Genetic
Show More
Related Content
More details
DOI :
10.1128/AEM.02889-05
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21094
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
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Scientific Publication
Analysis of quantitative interactions between two species of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices, by real-time PCR
72
Alkan, N., Department of Agronomy and Natural Resources, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Gadkar, V., Microbial Ecology Program, Division of Biological Sciences, University of Montana, 32 Campus Dr. 4824, Missoula, MT 59812, United States
Yarden, Q., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76 100, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel
Analysis of quantitative interactions between two species of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices, by real-time PCR
Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs, known to play an important role in ecological processes. Conventional light microscopy is the most common method used to detect their presence in planta, but this method fails to discern the presence of multiple AMF species and is not quantitative. These two factors are critically important in ecological studies, where the symbiotic contribution of each isolate needs to be defined. This paper describes the use of quantitative real-time PCR (qRT-PCR) as a detection system to address this issue. We used two Glomus spp., namely, G. intraradices and G. mosseae, to show that it is possible to study the interactions between these two isolates during the cocolonization of a single root system. Three different physiological studies were set up to assess how the interactions affected the occupancy of these fungi intraradically on a temporal basis. These treatments included saline and phosphorus stress, spatial distribution in the root zone, and preference for a particular host. qRT-PCR could prove a valuable tool in the area of AMF field ecology, where such data are critically important for defining the role of each species in the community structure. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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