Moen, T., AKVAFORSK, Aas, Norway
Agresti, J.J., Department of Animal Science, University of California, Davis, CA, United States, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
Cnaani, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel, Hubbard Center for Genome Studies, University of New Hampshire, Durham, United States
Moses, H., Department of Animal Science, University of California, Davis, CA, United States
Famula, T.R., Department of Animal Science, University of California, Davis, CA, United States
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Gall, G.A.E., Department of Animal Science, University of California, Davis, CA, United States
May, B., Department of Animal Science, University of California, Davis, CA, United States, Department of Animal Science, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States
Agresti, J.J., Department of Animal Science, University of California, Davis, CA, United States, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
Cnaani, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel, Hubbard Center for Genome Studies, University of New Hampshire, Durham, United States
Moses, H., Department of Animal Science, University of California, Davis, CA, United States
Famula, T.R., Department of Animal Science, University of California, Davis, CA, United States
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Gall, G.A.E., Department of Animal Science, University of California, Davis, CA, United States
May, B., Department of Animal Science, University of California, Davis, CA, United States, Department of Animal Science, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States
A genome scan, searching for quantitative trait loci (QTL) for the traits cold tolerance and body weight in tilapia, was performed on a cross between a (Oreochromis niloticus x Sarotherodon galilaeus) male and a (O. mossambicus x O. aureus) female. Fifty-four microsatellites and 23 amplified fragment length polymorphism (AFLP) primer combinations were genotyped and tested for marker-trait associations. Sex-specific linkage maps were constructed from this data. Twenty-three point-wise significant marker-trait associations were found in the genome scan, and putative QTL were subsequently tested in another (On x Sg) x (Om x Oa) family. None of the putative QTL from the first experiment were significant in the second experiment. However, one microsatellite, UNH130, found to be associated to weight in the first experiment, was found to be strongly associated to cold tolerance in the second experiment. Since QTL for cold tolerance and body weight were recently found on the linkage group containing UNH130 (linkage group 23) in another study, this linkage group was investigated more closely using interval mapping. The results provide indications, but not conclusive evidence, of a QTL for cold tolerance on linkage group 23.
