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Production of hydrogen peroxide and expression of ROS-generating genes in peach flower petals in response to host and non-host fungal pathogens
Year:
2013
Source of publication :
Plant Pathology
Authors :
Droby, Samir
;
.
Hershkovitz, Vera
;
.
Volume :
62
Co-Authors:

Liu, J., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Macarisin, D., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Wisniewski, M., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Sui, Y., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Norelli, J., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Hershkovitz, V., Agricultural Research Organization (ARO), Volcani Center, PO Box 6, Bet Dagan, 50250, Israel

Facilitators :
From page:
820
To page:
828
(
Total pages:
9
)
Abstract:
Reactive oxygen species (ROS) play dual roles in plant-microbe interactions in that they can either stimulate host resistance or enhance pathogen virulence. Innate resistance in peach (Prunus persica) to the brown rot fungal pathogen Monilinia fructicola is very limited, and knowledge of the mechanism of virulence is rudimentary. In this study, production of hydrogen peroxide, a major component of ROS, was determined in peach flower petals in response to M. fructicola (a host pathogen) and Penicillium digitatum (a non-host pathogen). Monilinia fructicola was able to infect flower petals while P. digitatum was not. During the host-specific interaction, M. fructicola induced hydrogen peroxide accumulation in flower petals. Application of exogenous antioxidants significantly reduced hydrogen peroxide accumulation as well as the incidence of brown rot disease. Application of M. fructicola spores to the surface of intact flower petals induced gene expression and increased enzyme activity of NADPH oxidase and cell wall peroxidase in host tissues, resulting in the production of hydrogen peroxide. Petals inoculated with M. fructicola exhibited high levels of protein carbonylation and lipid peroxidation. No significant response in gene expression, enzyme activity or hydrogen peroxide levels was observed in peach flower petals treated with P. digitatum. These results suggest that M. fructicola, as with other necrotrophic fungi, uses the strong oxidative response as part of a virulence mechanism. © 2012 The Authors Plant Pathology © 2012 BSPP.
Note:
Related Files :
disease resistance
fungi
gene expression
host specificity
Inoculation
Prunus persica
reactive oxygen species
virulence
Show More
Related Content
More details
DOI :
10.1111/j.1365-3059.2012.02683.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21159
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
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Scientific Publication
Production of hydrogen peroxide and expression of ROS-generating genes in peach flower petals in response to host and non-host fungal pathogens
62

Liu, J., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Macarisin, D., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Wisniewski, M., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Sui, Y., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Norelli, J., US Department of Agriculture, Agricultural Research Service, 2217 Wiltshire Road, Kearneysville, WV, 25430, United States
Hershkovitz, V., Agricultural Research Organization (ARO), Volcani Center, PO Box 6, Bet Dagan, 50250, Israel

Production of hydrogen peroxide and expression of ROS-generating genes in peach flower petals in response to host and non-host fungal pathogens
Reactive oxygen species (ROS) play dual roles in plant-microbe interactions in that they can either stimulate host resistance or enhance pathogen virulence. Innate resistance in peach (Prunus persica) to the brown rot fungal pathogen Monilinia fructicola is very limited, and knowledge of the mechanism of virulence is rudimentary. In this study, production of hydrogen peroxide, a major component of ROS, was determined in peach flower petals in response to M. fructicola (a host pathogen) and Penicillium digitatum (a non-host pathogen). Monilinia fructicola was able to infect flower petals while P. digitatum was not. During the host-specific interaction, M. fructicola induced hydrogen peroxide accumulation in flower petals. Application of exogenous antioxidants significantly reduced hydrogen peroxide accumulation as well as the incidence of brown rot disease. Application of M. fructicola spores to the surface of intact flower petals induced gene expression and increased enzyme activity of NADPH oxidase and cell wall peroxidase in host tissues, resulting in the production of hydrogen peroxide. Petals inoculated with M. fructicola exhibited high levels of protein carbonylation and lipid peroxidation. No significant response in gene expression, enzyme activity or hydrogen peroxide levels was observed in peach flower petals treated with P. digitatum. These results suggest that M. fructicola, as with other necrotrophic fungi, uses the strong oxidative response as part of a virulence mechanism. © 2012 The Authors Plant Pathology © 2012 BSPP.
Scientific Publication
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