Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Molecular diagnostic procedures for production of pathogen-free propagation material
Year:
2002
Source of publication :
Pest Management Science
Authors :
Chalupowicz, Laura
;
.
Dror, Orit
;
.
Kleitman, Frida
;
.
Volume :
58
Co-Authors:
Manulis, S., Department of Plant Pathology, ARO, Volcani Center, Bet-Dagan, 50250, Israel
Chalupowicz, L., Department of Plant Pathology, ARO, Volcani Center, Bet-Dagan, 50250, Israel
Dror, O., Department of Plant Pathology, ARO, Volcani Center, Bet-Dagan, 50250, Israel
Kleitman, F., Department of Plant Pathology, ARO, Volcani Center, Bet-Dagan, 50250, Israel
Facilitators :
From page:
1126
To page:
1131
(
Total pages:
6
)
Abstract:
Production of disease-free propagation material is a major means of controlling most bacterial diseases of plants, particularly when neither resistant clones nor effective chemical treatments are available. For this purpose sensitive, specific and rapid detection methods are required. The advent of molecular biology and, in particular, the polymerase chain reaction (PCR) has opened new ways for the characterization and identification of plant pathogens and the development of disease-management strategies. PCR-based detection methods rely on the development of primers for the specific detection of the pathogen. The use of pathogenicity genes as targets for primer design is the preferred procedure for obtaining specific primers but other procedures may also be useful for this purpose. In the present review we describe four examples of procedures for detecting four important bacterial pathogens in Israel: Erwinia herbicola pv gypsophilae in gypsophila, Xanthomonas campestris pv pelargonii in geranium, Agrobacterium tumefaciens in asters and roses, and Xanthomonas campestris pv campestris in crucifers. Procedures for constructing specific PCR primers for each bacterium are illustrated and discussed as well as the combination of PCR with other methods. © 2002 Society of Chemical Industry.
Note:
Related Files :
bacteria
Brassicaceae
Genetics
Geranium
Gypsophila
Israel
Rosa
Show More
Related Content
More details
DOI :
10.1002/ps.533
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
21160
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
You may also be interested in
Scientific Publication
נגישות
menu