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Virulence and the production of endo-1,4-β-glucanase by isolates of Alternaria alternata involved in the moldy-core disease of apples
Year:
2007
Source of publication :
Journal of Phytopathology
Authors :
Eshel, Dani
;
.
Prusky, Dov
;
.
Volume :
155
Co-Authors:
Reuveni, M., Golan Research Institute, University of Haifa, Katzrin, Israel, Golan Research Institute, University of Haifa, P.O. Box 97, Katzrin 12900, Israel
Sheglov, N., Golan Research Institute, University of Haifa, Katzrin, Israel, Golan Research Institute, University of Haifa, P.O. Box 97, Katzrin 12900, Israel
Eshel, D., Golan Research Institute, University of Haifa, Katzrin, Israel, Department of Postharvest Science of Fresh Produce, Institute of Agricultural Products, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Golan Research Institute, University of Haifa, Katzrin, Israel, Department of Postharvest Science of Fresh Produce, Institute of Agricultural Products, Volcani Center, Bet Dagan 50250, Israel
Ben-Arie, R., Golan Research Institute, University of Haifa, Katzrin, Israel, Fruit Storage Research Laboratory, Kiryat Shmona, Israel
Facilitators :
From page:
50
To page:
55
(
Total pages:
6
)
Abstract:
Alternaria alternata, is the predominant fungal pathogen responsible for moldy-core in apple cultivars of the Red Delicious group. Here we report on the association between virulence of natural isolates of A. alternata, their production of endo-1,4-β-glucanase (EG) and moldy-core development in apple fruits. Based on decay development following wound inoculations of mature fruits, three of 150 isolates, collected in three orchards in northern Israel and representing low, moderate and high virulence, were selected and designated Rm44, Er30 and Sh42, respectively. All three isolates secreted EG when grown on enzyme-inducing medium (EIM) containing commercial cellulose or apple cell walls and this production was related to their degree of virulence. Polyacrylamide gel electrophoresis (PAGE) revealed quantitative differences between the three isolates, relative to their virulence. When fungal extracts were run in native gels, a single band with a molecular mass of 23 kDa showing EG activity was produced by the high- (Sh42) and the medium-virulence (Er30) isolate but not by the low-virulence (Rm44) isolate. A commercial cellulase preparation (containing endo- and exo-1,4-β-glucanase) placed on pricked fruit led to the formation of symptoms similar to those developing on A. alternata-inoculated fruits within 2-4 days. Inoculation of bloom clusters at full bloom with the highly virulent isolate (Sh42) of A. alternata resulted in a significantly higher infection in fruits (58%) than in those inoculated with the low-virulence isolate (Rm44) (30%). Our results suggest that the moldy-core symptoms caused by A. alternata in apple, could be related to the ability of the fungus to produce EG in developing lesions. © 2007 The Authors.
Note:
Related Files :
Alternaria
Endoglucanase
enzyme activity
Eurasia
fungi
Israel
Malus x domestica
Middle East
Moldy-core disease
virulence
Show More
Related Content
More details
DOI :
10.1111/j.1439-0434.2006.01201.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21172
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
You may also be interested in
Scientific Publication
Virulence and the production of endo-1,4-β-glucanase by isolates of Alternaria alternata involved in the moldy-core disease of apples
155
Reuveni, M., Golan Research Institute, University of Haifa, Katzrin, Israel, Golan Research Institute, University of Haifa, P.O. Box 97, Katzrin 12900, Israel
Sheglov, N., Golan Research Institute, University of Haifa, Katzrin, Israel, Golan Research Institute, University of Haifa, P.O. Box 97, Katzrin 12900, Israel
Eshel, D., Golan Research Institute, University of Haifa, Katzrin, Israel, Department of Postharvest Science of Fresh Produce, Institute of Agricultural Products, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Golan Research Institute, University of Haifa, Katzrin, Israel, Department of Postharvest Science of Fresh Produce, Institute of Agricultural Products, Volcani Center, Bet Dagan 50250, Israel
Ben-Arie, R., Golan Research Institute, University of Haifa, Katzrin, Israel, Fruit Storage Research Laboratory, Kiryat Shmona, Israel
Virulence and the production of endo-1,4-β-glucanase by isolates of Alternaria alternata involved in the moldy-core disease of apples
Alternaria alternata, is the predominant fungal pathogen responsible for moldy-core in apple cultivars of the Red Delicious group. Here we report on the association between virulence of natural isolates of A. alternata, their production of endo-1,4-β-glucanase (EG) and moldy-core development in apple fruits. Based on decay development following wound inoculations of mature fruits, three of 150 isolates, collected in three orchards in northern Israel and representing low, moderate and high virulence, were selected and designated Rm44, Er30 and Sh42, respectively. All three isolates secreted EG when grown on enzyme-inducing medium (EIM) containing commercial cellulose or apple cell walls and this production was related to their degree of virulence. Polyacrylamide gel electrophoresis (PAGE) revealed quantitative differences between the three isolates, relative to their virulence. When fungal extracts were run in native gels, a single band with a molecular mass of 23 kDa showing EG activity was produced by the high- (Sh42) and the medium-virulence (Er30) isolate but not by the low-virulence (Rm44) isolate. A commercial cellulase preparation (containing endo- and exo-1,4-β-glucanase) placed on pricked fruit led to the formation of symptoms similar to those developing on A. alternata-inoculated fruits within 2-4 days. Inoculation of bloom clusters at full bloom with the highly virulent isolate (Sh42) of A. alternata resulted in a significantly higher infection in fruits (58%) than in those inoculated with the low-virulence isolate (Rm44) (30%). Our results suggest that the moldy-core symptoms caused by A. alternata in apple, could be related to the ability of the fungus to produce EG in developing lesions. © 2007 The Authors.
Scientific Publication
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