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אסיף מאגר המחקר החקלאי
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The operon for cytokinin biosynthesis of Erwinia herbicola pv. gypsophilae contains two promoters and is plant induced
Year:
2001
Source of publication :
Canadian Journal of Microbiology
Authors :
Lichter, Amnon
;
.
Manulis-Sasson, Shulamit
;
.
Volume :
47
Co-Authors:

Guo, M., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis, S.
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Lichter, A.

Facilitators :
From page:
1126
To page:
1131
(
Total pages:
6
)
Abstract:
The operon for cytokinin biosynthesis in the gall-forming bacterium Erwinia herbicola pv. gypsophilae (Ehg) has been previously shown to reside on an indigenous plasmid (pPATHEhg) that is mandatory for pathogenicity. This operon consists of two genes: the first open reading frame (pre-etz) is of unknown function, whereas the second one (etz) encodes for isopentenyl transferase. Northern hybridization performed with the wild-type strain Ehg824-1 grown in Luria-Bertani broth demonstrated two transcripts of which an etz-specific transcript (1.0 kb) was predominant. Fusion of upstream DNA fragments of both pre-etz and etz to the ice nucleation reporter gene inaZ in pVSP61 showed high ice nucleation activity in both cultures, confirming the presence of two independent promoters. An increase of 1-1.5 orders in transcriptional activity of these promoters was observed following inoculation of gypsophila cuttings. Mutants of Ehg824-1 were generated by insertion of inaZ into pre-etz and etz using the transposon reporter Tn3-Spice. An increase of about two orders in transcriptional activity was recorded with both mutants following inoculation of gypsophila or bean cuttings. A similar induction was also observed when the bacteria were applied to the leaf surface of these plants. Unlike other virulence genes present on the pPATHEhg, neither pre-etz nor etz was regulated by the adjacent hrp gene cluster.
Note:

The correct link to the article:

https://www.nrcresearchpress.com/doi/abs/10.1139/w01-116#.XIevxChvaM8

Related Files :
DNA
genetic engineering
hybridization
mutation
Plants
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More details
DOI :
10.1139/cjm-47-12-1126
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21176
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
You may also be interested in
Scientific Publication
The operon for cytokinin biosynthesis of Erwinia herbicola pv. gypsophilae contains two promoters and is plant induced
47

Guo, M., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis, S.
Barash, I., Department of Plant Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Lichter, A.

The operon for cytokinin biosynthesis of Erwinia herbicola pv. gypsophilae contains two promoters and is plant induced
The operon for cytokinin biosynthesis in the gall-forming bacterium Erwinia herbicola pv. gypsophilae (Ehg) has been previously shown to reside on an indigenous plasmid (pPATHEhg) that is mandatory for pathogenicity. This operon consists of two genes: the first open reading frame (pre-etz) is of unknown function, whereas the second one (etz) encodes for isopentenyl transferase. Northern hybridization performed with the wild-type strain Ehg824-1 grown in Luria-Bertani broth demonstrated two transcripts of which an etz-specific transcript (1.0 kb) was predominant. Fusion of upstream DNA fragments of both pre-etz and etz to the ice nucleation reporter gene inaZ in pVSP61 showed high ice nucleation activity in both cultures, confirming the presence of two independent promoters. An increase of 1-1.5 orders in transcriptional activity of these promoters was observed following inoculation of gypsophila cuttings. Mutants of Ehg824-1 were generated by insertion of inaZ into pre-etz and etz using the transposon reporter Tn3-Spice. An increase of about two orders in transcriptional activity was recorded with both mutants following inoculation of gypsophila or bean cuttings. A similar induction was also observed when the bacteria were applied to the leaf surface of these plants. Unlike other virulence genes present on the pPATHEhg, neither pre-etz nor etz was regulated by the adjacent hrp gene cluster.

The correct link to the article:

https://www.nrcresearchpress.com/doi/abs/10.1139/w01-116#.XIevxChvaM8

Scientific Publication
You may also be interested in