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Adventive plants from ovules and nucelli in Citrus
Year:
1972
Source of publication :
Planta
Authors :
Kochba, Joshuah
;
.
Safran, Hannah
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
106
Co-Authors:
Kochba, J., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Spiegel-Roy, P., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Safran, H., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Facilitators :
From page:
237
To page:
245
(
Total pages:
9
)
Abstract:
1- to 8-week-old ovules and nucelli from three Citrus cultivars-Shamouti and Valencia (Citrus sinensis) oranges and Marsh Seedless (C. paradisi) grapefruit-were cultured in vitro. No embryo differentiation was observed in the explants prior to culture. The Shamouti ovules had degenerated and were apparently unfertilized. Embryoids formed on Murashige and Tucker nutrient medium supplemented with 500 mg/l malt extract. Whole plants developed on the same basal medium supplemented with kinetin and indole-3-acetic acid (IAA), coconut milk or gibberellic acid (GA3). A higher kinetin/IAA ratio or the addition of coconut milk favoured stem elongation more than root formation while a lower kinetin/IAA ratio favoured root formation and inhibited stem elongation. The addition of GA3 to the basal medium stimulated rooting and stem elongation. These results can be of aid in mutation research, allowing irradiation at stages prior to embryonic development. © 1972 Springer-Verlag.
Note:
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More details
DOI :
10.1007/BF00388101
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21215
Last updated date:
18/12/2023 13:55
Creation date:
16/04/2018 23:42
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Scientific Publication
Adventive plants from ovules and nucelli in Citrus
106
Kochba, J., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Spiegel-Roy, P., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Safran, H., The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Adventive plants from ovules and nucelli in Citrus
1- to 8-week-old ovules and nucelli from three Citrus cultivars-Shamouti and Valencia (Citrus sinensis) oranges and Marsh Seedless (C. paradisi) grapefruit-were cultured in vitro. No embryo differentiation was observed in the explants prior to culture. The Shamouti ovules had degenerated and were apparently unfertilized. Embryoids formed on Murashige and Tucker nutrient medium supplemented with 500 mg/l malt extract. Whole plants developed on the same basal medium supplemented with kinetin and indole-3-acetic acid (IAA), coconut milk or gibberellic acid (GA3). A higher kinetin/IAA ratio or the addition of coconut milk favoured stem elongation more than root formation while a lower kinetin/IAA ratio favoured root formation and inhibited stem elongation. The addition of GA3 to the basal medium stimulated rooting and stem elongation. These results can be of aid in mutation research, allowing irradiation at stages prior to embryonic development. © 1972 Springer-Verlag.
Scientific Publication
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