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Differentiating PVYNTN from PVYN by annealing to reference RNA transcripts
Year:
2001
Source of publication :
Journal of Virological Methods
Authors :
Maslenin, Ludmila
;
.
Rosner, Arie
;
.
Volume :
97
Co-Authors:
Rosner, A., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
125
To page:
131
(
Total pages:
7
)
Abstract:
A method for the differentiation of virus strains based on the shift in electrophoretic mobility of partially annealed RNA transcripts is described. Oppositely oriented RNA transcripts of the NTN- and N-strains of PVY, complementary at their 3′-end variable (strain-specific) region, were annealed to form a partial duplex which moved more slowly in gel than heterologous (NTN + N) unpaired transcripts. Thus, the two virus strains could be identified by annealing to a known reference transcript. The rate of duplex migration was correlated with transcript lengths and could be tightly controlled thereby. Thus, a higher degree of resolution was obtained than with transcript conformation polymorphism, which is empirical and unpredictable in nature. © 2001 Elsevier Science B.V. All rights reserved.
Note:
Related Files :
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Solanum tuberosum
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Variation (Genetics)
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More details
DOI :
10.1016/S0166-0934(01)00345-7
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21217
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
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Scientific Publication
Differentiating PVYNTN from PVYN by annealing to reference RNA transcripts
97
Rosner, A., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Differentiating PVYNTN from PVYN by annealing to reference RNA transcripts
A method for the differentiation of virus strains based on the shift in electrophoretic mobility of partially annealed RNA transcripts is described. Oppositely oriented RNA transcripts of the NTN- and N-strains of PVY, complementary at their 3′-end variable (strain-specific) region, were annealed to form a partial duplex which moved more slowly in gel than heterologous (NTN + N) unpaired transcripts. Thus, the two virus strains could be identified by annealing to a known reference transcript. The rate of duplex migration was correlated with transcript lengths and could be tightly controlled thereby. Thus, a higher degree of resolution was obtained than with transcript conformation polymorphism, which is empirical and unpredictable in nature. © 2001 Elsevier Science B.V. All rights reserved.
Scientific Publication
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