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Role of ammonia secretion and pH modulation on pathogenicity of Colletotrichum coccodes on tomato fruit
Year:
2008
Authors :
Alkan, Noam
;
.
Prusky, Dov
;
.
Sherman, Amir
;
.
Volume :
21
Co-Authors:
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Sherman, A., Department of Genomics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1058
To page:
1066
(
Total pages:
9
)
Abstract:
Colletotrichum coccodes was found to alkalinize the decaying tissue of tomato fruit via accumulation and secretion of ammonia. Alkalinization dynamics caused by ammonia secretion from growing hyphae was examined microscopically using the pH-sensitive fluorescent dye 2′,7′-bis(carboxyethyl)-5(6)- carboxyfluorescein. Values of pH of 7.9 observed in the host tissue close to the hyphal tips declined to pH 6.0 at 10 mm away from the hyphal tip, which was a value that was still higher than that detected in the healthy tissue, pH 4.2. Ammonia accumulation at the infection site depended on the initial environmental pH. Treatments with low (4.0) pH buffer at the infection site resulted in high levels of ammonia secretion and increased virulence of C. coccodes compared with similar treatments with buffer at pH 7.0. Significantly, mutants of C. coccodes defective in nitrogen utilization, nit-, and areA- were impaired in ammonia secretion and showed reduced decay development. The reduced infection rate of nit- mutants could be complemented by adding glutamine at the infection site. Thus, ammonia accumulation is a critical factor contributing to C. coccodes pathogenicity on tomato fruit. The results show that the initial acidic pH of the fruit is conducive to ammonia secretion and the subsequent alkalinization of the infection site, and facilitates fungal virulence and the transformation from the quiescent-biotrophic to active-necrotrophic state. © 2008 The American Phytopathological Society.
Note:
Related Files :
Colletotrichum
fungi
metabolism
Microbiology
pH
Plant Disease
Plant Diseases
virulence
Show More
Related Content
More details
DOI :
10.1094/MPMI-21-8-1058
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21221
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
You may also be interested in
Scientific Publication
Role of ammonia secretion and pH modulation on pathogenicity of Colletotrichum coccodes on tomato fruit
21
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Sherman, A., Department of Genomics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Role of ammonia secretion and pH modulation on pathogenicity of Colletotrichum coccodes on tomato fruit
Colletotrichum coccodes was found to alkalinize the decaying tissue of tomato fruit via accumulation and secretion of ammonia. Alkalinization dynamics caused by ammonia secretion from growing hyphae was examined microscopically using the pH-sensitive fluorescent dye 2′,7′-bis(carboxyethyl)-5(6)- carboxyfluorescein. Values of pH of 7.9 observed in the host tissue close to the hyphal tips declined to pH 6.0 at 10 mm away from the hyphal tip, which was a value that was still higher than that detected in the healthy tissue, pH 4.2. Ammonia accumulation at the infection site depended on the initial environmental pH. Treatments with low (4.0) pH buffer at the infection site resulted in high levels of ammonia secretion and increased virulence of C. coccodes compared with similar treatments with buffer at pH 7.0. Significantly, mutants of C. coccodes defective in nitrogen utilization, nit-, and areA- were impaired in ammonia secretion and showed reduced decay development. The reduced infection rate of nit- mutants could be complemented by adding glutamine at the infection site. Thus, ammonia accumulation is a critical factor contributing to C. coccodes pathogenicity on tomato fruit. The results show that the initial acidic pH of the fruit is conducive to ammonia secretion and the subsequent alkalinization of the infection site, and facilitates fungal virulence and the transformation from the quiescent-biotrophic to active-necrotrophic state. © 2008 The American Phytopathological Society.
Scientific Publication
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