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High Performance Liquid Chromatographic Identification of Anthocyanins
Year:
1980
Authors :
Akavia, Naaman
;
.
Volume :
35
Co-Authors:
Strack, D., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
Akavia, N., Department of Floriculture and Ornamental Plants, Volcani Center, Bet Dagan 50-200, Israel
Reznik, H., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
Facilitators :
From page:
533
To page:
538
(
Total pages:
6
)
Abstract:
A high performance liquid chromatographic procedure for a rapid identification of antho-cyanins is described. Besides their characteristic retention times, anthocyanins can easily be identified by an HPLC examination of the kinetics of appearance and disappearance of their partial hydrolysis products. This method is also applicable to 3-deoxyanthocyanins which is demonstrated with an extract from the fern Blechnum brasiliense. The described method allows optimal resolution of complex anthocyanin mixtures in the following sequence of elution: 3-sophoroside-5-glucoside; 3-rutinoside-5-glucoside; 3, 5-diglucoside; 3-sophoroside; 3-galactoside; 3-glucoside; 3-rutinoside; 5-glucoside; aglycone, and finally the forms acylated with p-coumaric acid. Substitution in the B-ring of the aglycone structure is the key factor for the range of the retention time. Thus cyanidin 3, 5-diglucoside elutes earlier (—38 sec) than pelargonidin 3-sophoroside-5-glucoside. © 1980, Walter de Gruyter. All rights reserved.
Note:
Related Files :
3-Deoxyanthocyanins
Anthocyanins
high performance liquid chromatography
Partial Hydrolysis
Show More
Related Content
More details
DOI :
10.1515/znc-1980-7-802
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21230
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
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Scientific Publication
High Performance Liquid Chromatographic Identification of Anthocyanins
35
Strack, D., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
Akavia, N., Department of Floriculture and Ornamental Plants, Volcani Center, Bet Dagan 50-200, Israel
Reznik, H., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
High Performance Liquid Chromatographic Identification of Anthocyanins
A high performance liquid chromatographic procedure for a rapid identification of antho-cyanins is described. Besides their characteristic retention times, anthocyanins can easily be identified by an HPLC examination of the kinetics of appearance and disappearance of their partial hydrolysis products. This method is also applicable to 3-deoxyanthocyanins which is demonstrated with an extract from the fern Blechnum brasiliense. The described method allows optimal resolution of complex anthocyanin mixtures in the following sequence of elution: 3-sophoroside-5-glucoside; 3-rutinoside-5-glucoside; 3, 5-diglucoside; 3-sophoroside; 3-galactoside; 3-glucoside; 3-rutinoside; 5-glucoside; aglycone, and finally the forms acylated with p-coumaric acid. Substitution in the B-ring of the aglycone structure is the key factor for the range of the retention time. Thus cyanidin 3, 5-diglucoside elutes earlier (—38 sec) than pelargonidin 3-sophoroside-5-glucoside. © 1980, Walter de Gruyter. All rights reserved.
Scientific Publication
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