אסיף מאגר המחקר החקלאי

Isolation of acidic and conjugated ecdysteroid fractions from Manduca sexta pupae

Year:

1988

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Volume :

18

Co-Authors:

Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States

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Abstract:

Radiolabeled polar ecdysteroids were isolated from Manduca sexta pupae and applied to a column of the ion-exchange adsorbent DEAE-Sephadex. Elution with a NaCl gradient solvent system efficiently separated the polar ecdysteroids into three groups, eluted in order of their increasing ionic strength: free ecdysteroids, ecdysteroid acids, and ecdysteroid conjugates. The separation method afforded excellent sample recovery and purification, and facilitated HPLC analysis of the ecdysteroid acids which may be key inactivation products of molting hormones in M. sexta. Isolation procedures are described in detail. © 1988.

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