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Isolation of acidic and conjugated ecdysteroid fractions from Manduca sexta pupae
Year:
1988
Source of publication :
Insect Biochemistry
Authors :
Svoboda, James
;
.
Volume :
18
Co-Authors:
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Facilitators :
From page:
163
To page:
168
(
Total pages:
6
)
Abstract:
Radiolabeled polar ecdysteroids were isolated from Manduca sexta pupae and applied to a column of the ion-exchange adsorbent DEAE-Sephadex. Elution with a NaCl gradient solvent system efficiently separated the polar ecdysteroids into three groups, eluted in order of their increasing ionic strength: free ecdysteroids, ecdysteroid acids, and ecdysteroid conjugates. The separation method afforded excellent sample recovery and purification, and facilitated HPLC analysis of the ecdysteroid acids which may be key inactivation products of molting hormones in M. sexta. Isolation procedures are described in detail. © 1988.
Note:
Related Files :
DEAE-Sephadex
ecdysteroid acids
ecdysteroid conjugates
ion-exchange column chromatography
Manduca sexta
Show More
Related Content
More details
DOI :
10.1016/0020-1790(88)90020-0
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21241
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
You may also be interested in
Scientific Publication
Isolation of acidic and conjugated ecdysteroid fractions from Manduca sexta pupae
18
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States
Isolation of acidic and conjugated ecdysteroid fractions from Manduca sexta pupae
Radiolabeled polar ecdysteroids were isolated from Manduca sexta pupae and applied to a column of the ion-exchange adsorbent DEAE-Sephadex. Elution with a NaCl gradient solvent system efficiently separated the polar ecdysteroids into three groups, eluted in order of their increasing ionic strength: free ecdysteroids, ecdysteroid acids, and ecdysteroid conjugates. The separation method afforded excellent sample recovery and purification, and facilitated HPLC analysis of the ecdysteroid acids which may be key inactivation products of molting hormones in M. sexta. Isolation procedures are described in detail. © 1988.
Scientific Publication
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