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Actomyosin mediates gravisensing and early transduction events in reoriented cut snapdragon spikes
Year:
2011
Source of publication :
Journal of Plant Physiology
Authors :
Belausov, Eduard
;
.
Friedman, Haya
;
.
Meir, Shimon
;
.
Philosoph-Hadas, Sonia
;
.
Volume :
168
Co-Authors:
Zhang, Z., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel, College of Horticulture, South China Agricultural University, 510642 Guangzhou, China
Friedman, H., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Meir, S., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Belausov, E., Department of Ornamentals, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
1176
To page:
1183
(
Total pages:
8
)
Abstract:
We investigated the involvement of the actomyosin network in the early events of the gravitropic response of cut snapdragon (Antirrhinum majus L.) spikes. The effects of the actin-modulating drug, cytochalasin D (CD) and/or the myosin inhibitor, 2,3-butanedione-2-monoxime (BDM) on amyloplast displacement, lateral auxin transport and consequently on stem bending were examined. The inhibitory effect on cytoskeleton integrity was studied by using indirect immunofluorescence double-labeling of actin and myosin. Our results demonstrate that no organizational changes in actin filaments occurred in cortical and endodermal cells of the stem bending zone during reorientation. These results suggest that actin depolymerization is not required for amyloplast sedimentation. Unlike the chloroplasts in the cortex, the amyloplasts in the endodermis were surrounded by actin and myosin, indicating that amyloplasts may be attached to the actin filaments via the motor protein, myosin. This suggests the involvement of myosin as part of the actomyosin complex in amyloplast movement in vertical as well as in reoriented stems. This suggestion was supported by the findings showing that: (a) BDM or CD disrupted the normal organization of actin either by altering characteristic distribution patterns of myosin-like protein in the cortex (BDM), or by causing actin fragmentation (CD); (b) both compounds inhibited the gravity-induced amyloplast displacement in the endodermis. Additionally, these compounds also inhibited lateral auxin transport across the stem and stem gravitropic bending. Our study suggests that during stem reorientation amyloplasts possibly remain attached to the actin filaments, using myosin as a motor protein. Thus, gravisensing and early transduction events in the gravitropic response of snapdragon spikes, manifested by amyloplast displacement and lateral auxin transport, are mediated by the actomyosin complex. © 2011 Elsevier GmbH.
Note:
Related Files :
Antirrhinum
Antirrhinum majus
drug derivative
drug effect
Growth, Development and Aging
metabolism
Plastids
Shoot reorientation
Show More
Related Content
More details
DOI :
10.1016/j.jplph.2011.01.019
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21242
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
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Scientific Publication
Actomyosin mediates gravisensing and early transduction events in reoriented cut snapdragon spikes
168
Zhang, Z., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel, College of Horticulture, South China Agricultural University, 510642 Guangzhou, China
Friedman, H., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Meir, S., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Belausov, E., Department of Ornamentals, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Actomyosin mediates gravisensing and early transduction events in reoriented cut snapdragon spikes
We investigated the involvement of the actomyosin network in the early events of the gravitropic response of cut snapdragon (Antirrhinum majus L.) spikes. The effects of the actin-modulating drug, cytochalasin D (CD) and/or the myosin inhibitor, 2,3-butanedione-2-monoxime (BDM) on amyloplast displacement, lateral auxin transport and consequently on stem bending were examined. The inhibitory effect on cytoskeleton integrity was studied by using indirect immunofluorescence double-labeling of actin and myosin. Our results demonstrate that no organizational changes in actin filaments occurred in cortical and endodermal cells of the stem bending zone during reorientation. These results suggest that actin depolymerization is not required for amyloplast sedimentation. Unlike the chloroplasts in the cortex, the amyloplasts in the endodermis were surrounded by actin and myosin, indicating that amyloplasts may be attached to the actin filaments via the motor protein, myosin. This suggests the involvement of myosin as part of the actomyosin complex in amyloplast movement in vertical as well as in reoriented stems. This suggestion was supported by the findings showing that: (a) BDM or CD disrupted the normal organization of actin either by altering characteristic distribution patterns of myosin-like protein in the cortex (BDM), or by causing actin fragmentation (CD); (b) both compounds inhibited the gravity-induced amyloplast displacement in the endodermis. Additionally, these compounds also inhibited lateral auxin transport across the stem and stem gravitropic bending. Our study suggests that during stem reorientation amyloplasts possibly remain attached to the actin filaments, using myosin as a motor protein. Thus, gravisensing and early transduction events in the gravitropic response of snapdragon spikes, manifested by amyloplast displacement and lateral auxin transport, are mediated by the actomyosin complex. © 2011 Elsevier GmbH.
Scientific Publication
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