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High expression of transgene protein in Spirodela
Year:
2007
Source of publication :
Plant Cell Reports
Authors :
Flaishman, Moshe
;
.
Perl, Avi
;
.
Volume :
26
Co-Authors:
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Li, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Hanania, U., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Flaishman, M., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Wisniewski, J.-P., LemnaGene SA, Lyon, France
Freyssinet, G., LemnaGene SA, Lyon, France
Facilitators :
From page:
1511
To page:
1519
(
Total pages:
9
)
Abstract:
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant. © 2007 Springer-Verlag.
Note:
Related Files :
Biomass
Genetics
genetic transformation
Genome
metabolism
mutation
proteins
tissue culture
Show More
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More details
DOI :
10.1007/s00299-007-0361-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21245
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
Scientific Publication
High expression of transgene protein in Spirodela
26
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Li, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Hanania, U., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Flaishman, M., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Wisniewski, J.-P., LemnaGene SA, Lyon, France
Freyssinet, G., LemnaGene SA, Lyon, France
High expression of transgene protein in Spirodela
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant. © 2007 Springer-Verlag.
Scientific Publication
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