Co-Authors:
Vunsh, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Li, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Hanania, U., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Flaishman, M., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, ARO Volcani Center, Bet Dagan, Israel
Wisniewski, J.-P., LemnaGene SA, Lyon, France
Freyssinet, G., LemnaGene SA, Lyon, France
Abstract:
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant. © 2007 Springer-Verlag.
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