Holland, D., Department of Fruit Tree Breeding and Genetics, Agricultural Research Organisation, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Abied, M.A., Department of Fruit Tree Breeding and Genetics, Agricultural Research Organisation, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Nachman, S., Department of Fruit Tree Breeding and Genetics, Agricultural Research Organisation, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Saad, S., Department of Fruit Tree Breeding and Genetics, Agricultural Research Organisation, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel

The role of cotyledons in seedling development and precocious flowering was studied in 'Duncan' grapefruit (Citrus paradisi Macf), a cultivar that displays a high frequency of precocious flowering. Cotyledons were detached from the embryo and the embryos were germinated in vitro to form plantlets. Cotyledon detachment dramatically affected the development of 'Duncan' seedlings. The decotyledonized plants were stunted, with small narrow leaves and thin and underdeveloped roots. Decotyledonization did not change significantly the number of leaves developed. Despite the dramatic effects of the cotyledons on seedling development, decotyledonized 'Duncan' seedlings retained their ability to flower precociously. We conclude that although normal growth and development of 'Duncan' grapefruit seedlings is cotyledon-dependent, the ability to flower precociously does not depend on the presence of cotyledons during in vitro germination. © 1995 Kluwer Academic Publishers.