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ACTH administration during formation of preovulatory follicles impairs steroidogenesis and angiogenesis in association with ovulation failure in lactating cows
Year:
2015
Source of publication :
Domestic Animal Endocrinology
Authors :
Braw-Tal, Ruth
;
.
Volume :
53
Co-Authors:
Biran, D., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Braw-Tal, R., Department of Ruminant Science, Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gendelman, M., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Lavon, Y., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Roth, Z., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Facilitators :
From page:
52
To page:
59
(
Total pages:
8
)
Abstract:
Ovulation failure, follicular persistence, and formation of follicular cysts are known to impair dairy cow fertility. Although the underlying mechanism is not entirely clear, stress-induced alteration in adrenal hormone secretion can cause these ovarian pathologies. Six synchronized lactating cows were scanned daily by ultrasound, and plasma samples were taken throughout the estrous cycle. Treatment cows (n = 3) were administered with ACTH analog every 12h from day 15 to day 21 of the cycle to induce formation of follicular cysts. Ovaries were collected at the slaughterhouse on day 23 of the cycle before appearance of follicular pathologies. Control cows (n = 3) were administered placebo, resynchronized, and administered PGF on day 6 of the new cycle to induce development of a preovulatory follicle. Follicular fluid was aspirated from the preovulatory follicles of each group to determine their steroid milieu. Slices were taken from the follicular wall for total messenger (m) RNA isolation and semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Administration of ACTH increased (P < 0.02) plasma cortisol concentration and reduced (P < 0.01) milk production. Androstenedione and estradiol concentrations in the follicular fluids were lower (P < 0.05) in ACTH-treated follicles than those in controls. The mRNA expression of luteinizing hormone receptor, 3β-hydroxysteroid dehydrogenase, cytochrome P450 aromatase (P450arom), and cytochrome P450 17α-hydroxylase (P450c17) were lower (P < 0.02) in the ACTH-treated vs control cows. On the other hand, the expression of steroidogenic acute regulatory protein and cytochrome P450 side-chain cleavage did not differ between groups. In addition, mRNA expression of vascular endothelial growth factor (VEGF)120 and VEGF164 was higher (P < 0.01) in control than in ACTH-treated follicles, but that for angiopoietin-1 and 2 did not differ between groups. Findings indicated that ACTH administration throughout preovulatory follicle development alters follicular steroidogenesis in association with impaired angiogenesis. Such alterations might explain, in part, the mechanism underlying ovulation failure and the formation of persistent or cystic follicles under stress. © 2015 Elsevier Inc..
Note:
Related Files :
Animal
Animals
Blood
cattle
Female
Genetics
lactation
metabolism
stress
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Related Content
More details
DOI :
10.1016/j.domaniend.2015.05.002
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21295
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:43
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Scientific Publication
ACTH administration during formation of preovulatory follicles impairs steroidogenesis and angiogenesis in association with ovulation failure in lactating cows
53
Biran, D., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Braw-Tal, R., Department of Ruminant Science, Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gendelman, M., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Lavon, Y., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Roth, Z., Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
ACTH administration during formation of preovulatory follicles impairs steroidogenesis and angiogenesis in association with ovulation failure in lactating cows
Ovulation failure, follicular persistence, and formation of follicular cysts are known to impair dairy cow fertility. Although the underlying mechanism is not entirely clear, stress-induced alteration in adrenal hormone secretion can cause these ovarian pathologies. Six synchronized lactating cows were scanned daily by ultrasound, and plasma samples were taken throughout the estrous cycle. Treatment cows (n = 3) were administered with ACTH analog every 12h from day 15 to day 21 of the cycle to induce formation of follicular cysts. Ovaries were collected at the slaughterhouse on day 23 of the cycle before appearance of follicular pathologies. Control cows (n = 3) were administered placebo, resynchronized, and administered PGF on day 6 of the new cycle to induce development of a preovulatory follicle. Follicular fluid was aspirated from the preovulatory follicles of each group to determine their steroid milieu. Slices were taken from the follicular wall for total messenger (m) RNA isolation and semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Administration of ACTH increased (P < 0.02) plasma cortisol concentration and reduced (P < 0.01) milk production. Androstenedione and estradiol concentrations in the follicular fluids were lower (P < 0.05) in ACTH-treated follicles than those in controls. The mRNA expression of luteinizing hormone receptor, 3β-hydroxysteroid dehydrogenase, cytochrome P450 aromatase (P450arom), and cytochrome P450 17α-hydroxylase (P450c17) were lower (P < 0.02) in the ACTH-treated vs control cows. On the other hand, the expression of steroidogenic acute regulatory protein and cytochrome P450 side-chain cleavage did not differ between groups. In addition, mRNA expression of vascular endothelial growth factor (VEGF)120 and VEGF164 was higher (P < 0.01) in control than in ACTH-treated follicles, but that for angiopoietin-1 and 2 did not differ between groups. Findings indicated that ACTH administration throughout preovulatory follicle development alters follicular steroidogenesis in association with impaired angiogenesis. Such alterations might explain, in part, the mechanism underlying ovulation failure and the formation of persistent or cystic follicles under stress. © 2015 Elsevier Inc..
Scientific Publication
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