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Dispersed cells prepared from human parathyroid glands: Distinct calcium sensitivity of adenomas vs. primary hyperplasia
Year:
1978
Authors :
Hurvitz, Shmuel (Animal science)
;
.
Volume :
46
Co-Authors:
Brown, E.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Brennan, M.F., Surgery Branch, National Cancer Institute, NIH, Bethesda, MD, United States
Hurwitz, S., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Windeck, R., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Marx, S.J., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Spiegel, A.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Koehler, J.O., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Gardner, D.G., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Aurbach, G.D., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Facilitators :
From page:
267
To page:
276
(
Total pages:
10
)
Abstract:
Dispersed parathyroid cells were prepared by digestion with collagenase and DNase of parathyroid glands of patients with adenoma or primary hyperplasia. Yields of 50-100 million cells/g of tissue were obtained and the cells were viable by morphologic (95-100% trypan blue exclusion) and functional criteria [linear release of parathyroid hormone (PTH) responsive to ambient calcium concentration]. Cells prepared from 7 of 9 hyperplastic and 8 of 12 adenomatous glands were suppressible 50% (52-90%) or more by calcium. Cells from hyperplastic glands, however, were suppressed by significantly lower concentrations of calcium than those from adenomas and resembled normal bovine parathyroid cells in this respect. This results suggested that a "set-point" error in calcium-regulated PTH release might contribute to the pathophysiology of parathyroid adenomas. Cells from 2 of 9 hyperplastic and 4 of 12 adenomatous glands, on the other hand, were suppressible less than 50% (0-48%) by high calcium concentrations, indicating that some abnormal parathyroid glands may function in a truly autonomous fashion. Preliminary immunologic studies showed that incubation media from dispersed cells reacted with either Nor C-terminal-directed antisera and in parallel with bPTH (1-84). In addition, elevated calcium concentrations suppressed immunoreactive PTH release from a preparation of dispersed cells similarly, whether measured by the N-or C-specific assay. © 1978 by The Endocrine Society.
Note:
Related Files :
adult
Cell Separation
endocrine system
Female
human cell
Male
Parathyroid Glands
parathyroid hyperplasia
Show More
Related Content
More details
DOI :
10.1210/jcem-46-2-267
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21346
Last updated date:
21/08/2022 07:45
Creation date:
16/04/2018 23:43
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Scientific Publication
Dispersed cells prepared from human parathyroid glands: Distinct calcium sensitivity of adenomas vs. primary hyperplasia
46
Brown, E.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Brennan, M.F., Surgery Branch, National Cancer Institute, NIH, Bethesda, MD, United States
Hurwitz, S., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Windeck, R., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Marx, S.J., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Spiegel, A.M., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Koehler, J.O., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Gardner, D.G., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Aurbach, G.D., Metabolic Diseases Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, MD, United States
Dispersed cells prepared from human parathyroid glands: Distinct calcium sensitivity of adenomas vs. primary hyperplasia
Dispersed parathyroid cells were prepared by digestion with collagenase and DNase of parathyroid glands of patients with adenoma or primary hyperplasia. Yields of 50-100 million cells/g of tissue were obtained and the cells were viable by morphologic (95-100% trypan blue exclusion) and functional criteria [linear release of parathyroid hormone (PTH) responsive to ambient calcium concentration]. Cells prepared from 7 of 9 hyperplastic and 8 of 12 adenomatous glands were suppressible 50% (52-90%) or more by calcium. Cells from hyperplastic glands, however, were suppressed by significantly lower concentrations of calcium than those from adenomas and resembled normal bovine parathyroid cells in this respect. This results suggested that a "set-point" error in calcium-regulated PTH release might contribute to the pathophysiology of parathyroid adenomas. Cells from 2 of 9 hyperplastic and 4 of 12 adenomatous glands, on the other hand, were suppressible less than 50% (0-48%) by high calcium concentrations, indicating that some abnormal parathyroid glands may function in a truly autonomous fashion. Preliminary immunologic studies showed that incubation media from dispersed cells reacted with either Nor C-terminal-directed antisera and in parallel with bPTH (1-84). In addition, elevated calcium concentrations suppressed immunoreactive PTH release from a preparation of dispersed cells similarly, whether measured by the N-or C-specific assay. © 1978 by The Endocrine Society.
Scientific Publication
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