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Overweight and CpG methylation of the Pomc promoter in offspring of high-fat-diet-fed dams are not "reprogrammed" by regular chow diet in rats
Year:
2014
Source of publication :
FASEB Journal
Authors :
Kisliouk, Tatiana
;
.
Meiri, Noam
;
.
Volume :
28
Co-Authors:
Marco, A., Faculty of Life Sciences, Bar Ilan University, Ramat-Gan, Israel, Gonda Brain Research Center, Bar Ilan University, Ramat-Gan, Israel
Kisliouk, T., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Tabachnik, T., Faculty of Life Sciences, Bar Ilan University, Ramat-Gan, Israel, Gonda Brain Research Center, Bar Ilan University, Ramat-Gan, Israel
Meiri, N., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Weller, A., Gonda Brain Research Center, Bar Ilan University, Ramat-Gan, Israel, Department of Psychology, Bar Ilan University, Ramat-Gan, Israel
Facilitators :
From page:
4148
To page:
4157
(
Total pages:
10
)
Abstract:
This study aimed to determine whether epigenetic malprogramming induced by high-fat diet (HFD) has an obesogenic effect on nonmated and mated female rats and their offspring. Further, it aimed to reprogram offspring's epigenetic malprogramming and phenotype by providing normal diet after weaning. Body weight (BW) was measured, and plasma and hypothalamic arcuate nuclei were collected for analysis of hormones, mRNA, and DNA CpG methylation of the promoter of Pomc, a key factor in control of food intake. In nonmated females, HFD decreased Pomc/leptin ratio by ∼38%. This finding was associated with Pomc promoter hypermethylation. While heavier during pregnancy, during lactation HFD dams showed sharper BW decrease (2.5-fold) and loss of Pomc promoter hypermethylation. Moreover, their weight loss was correlated with demethylation (r=-0.707) and with gadd45b mRNA expression levels (r=0.905). Even though offspring of HFD dams ate standard chow from weaning, they displayed increased BW, Pomc promoter hypermethylation, and vulnerability to HFD challenge (3-fold kilocalorie intake increase). These findings demonstrate a long-term effect of maternal HFD on CpG methylation of the Pomc promoter in the offspring, which was not reprogrammed by standard chow from weaning. Further, the results suggest a possible mechanism of demethylation of the Pomc promoter following pregnancy and lactation. © FASEB.
Note:
Related Files :
Animals
DNA
epigenetics
Female
Genetics
lactation
Male
phenotype
pregnancy
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More details
DOI :
10.1096/fj.14-255620
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21450
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
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