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An investigation of the storage and biosynthesis of phenylpropenes in sweet basil
Year:
2001
Source of publication :
Plant physiology (source)
Authors :
Lewinsohn, Efraim
;
.
Volume :
125
Co-Authors:

Gang, D.R., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Wang, J., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Dudareva, N., Horticulture Department, Purdue University, West Lafayette, Indiana 47907
Nam, K.H., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Simon, J.E., Department of Plant Science, Rutgers University, New Brunswick, New Jersey 08901
Lewinsohn, E.
Pichersky, E., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States

Facilitators :
From page:
539
To page:
555
(
Total pages:
17
)
Abstract:
Plants that contain high concentrations of the defense compounds of the phenylpropene class (eugenol, chavicol, and their derivatives) have been recognized since antiquity as important spices for human consumption (e.g. cloves) and have high economic value. Our understanding of the biosynthetic pathway that produces these compounds in the plant, however, has remained incomplete. Several lines of basil (Ocimum basilicum) produce volatile oils that contain essentially only one or two specific phenylpropene compounds. Like other members of the Lamiaceae, basil leaves possess on their surface two types of glandular trichomes, termed peltate and capitate glands. We demonstrate here that the volatile oil constituents eugenol and methylchavicol accumulate, respectively, in the peltate glands of basil lines SW (which produces essentially only eugenol) and EMX-1 (which produces essentially only methylchavicol). Assays for putative enzymes in the biosynthetic pathway leading to these phenylpropenes localized many of the corresponding enzyme activities almost exclusively to the peltate glands in leaves actively producing volatile oil. An analysis of an expressed sequence tag database from leaf peltate glands revealed that known genes for the phenylpropanoid pathway are expressed at very high levels in these structures, accounting for 13% of the total expressed sequence tags. An additional 14% of cDNAs encoded enzymes for the biosynthesis of S-adenosyl-methionine, an important substrate in the synthesis of many phenylpropenes. Thus, the peltate glands of basil appear to be highly specialized structures for the synthesis and storage of phenylpropenes, and serve as an excellent model system to study phenylpropene biosynthesis.
Note:
Related Files :
biochemical pathway
biotransformation
Ocimum basilicum
phenols
phenylpropene
Plant Leaves
s adenosylmethionine
Show More
Related Content
More details
DOI :
10.1104/pp.125.2.539
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21451
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
You may also be interested in
Scientific Publication
An investigation of the storage and biosynthesis of phenylpropenes in sweet basil
125

Gang, D.R., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Wang, J., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Dudareva, N., Horticulture Department, Purdue University, West Lafayette, Indiana 47907
Nam, K.H., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States
Simon, J.E., Department of Plant Science, Rutgers University, New Brunswick, New Jersey 08901
Lewinsohn, E.
Pichersky, E., Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, United States

An investigation of the storage and biosynthesis of phenylpropenes in sweet basil
Plants that contain high concentrations of the defense compounds of the phenylpropene class (eugenol, chavicol, and their derivatives) have been recognized since antiquity as important spices for human consumption (e.g. cloves) and have high economic value. Our understanding of the biosynthetic pathway that produces these compounds in the plant, however, has remained incomplete. Several lines of basil (Ocimum basilicum) produce volatile oils that contain essentially only one or two specific phenylpropene compounds. Like other members of the Lamiaceae, basil leaves possess on their surface two types of glandular trichomes, termed peltate and capitate glands. We demonstrate here that the volatile oil constituents eugenol and methylchavicol accumulate, respectively, in the peltate glands of basil lines SW (which produces essentially only eugenol) and EMX-1 (which produces essentially only methylchavicol). Assays for putative enzymes in the biosynthetic pathway leading to these phenylpropenes localized many of the corresponding enzyme activities almost exclusively to the peltate glands in leaves actively producing volatile oil. An analysis of an expressed sequence tag database from leaf peltate glands revealed that known genes for the phenylpropanoid pathway are expressed at very high levels in these structures, accounting for 13% of the total expressed sequence tags. An additional 14% of cDNAs encoded enzymes for the biosynthesis of S-adenosyl-methionine, an important substrate in the synthesis of many phenylpropenes. Thus, the peltate glands of basil appear to be highly specialized structures for the synthesis and storage of phenylpropenes, and serve as an excellent model system to study phenylpropene biosynthesis.
Scientific Publication
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