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High-throughput genomic DNA extraction protocol from tilapia's fin tissue
Year:
2006
Source of publication :
aquaculture (source)
Authors :
Hulata, Gideon
;
.
Ron, Micha
;
.
Zilberman, Noam
;
.
Volume :
255
Co-Authors:
Zilberman, N., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Reikhav, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
597
To page:
599
(
Total pages:
3
)
Abstract:
We developed a high throughput DNA extraction protocol from tilapia fin tissue that enables the use of 96-well plates using a fluid micro-dispenser robot, thus effectively decreasing the time, cost and handling error of the DNA preparation stage. The method is based on the DNA salting out procedure and all fluid transfers are designed for use with a micro dispenser robot. The criterion of success was defined as extraction of at least 2 μg of DNA, which enables at least 100 PCR reactions. A total of 3163 samples met this criterion (98%). The cost of the proposed in-house protocol is approximately ten-fold lower than the commercial alternatives. The suggested high-throughput extraction protocol may be adjusted by slight modifications to handle other tissues. © 2005 Elsevier B.V. All rights reserved.
Note:
Related Files :
aquatic environment
DNA
DNA extraction
Fin tissue
fish
genomics
High throughput
PCR
Tilapia
Tilapias
Show More
Related Content
More details
DOI :
10.1016/j.aquaculture.2005.11.042
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21453
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
High-throughput genomic DNA extraction protocol from tilapia's fin tissue
255
Zilberman, N., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Reikhav, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
High-throughput genomic DNA extraction protocol from tilapia's fin tissue
We developed a high throughput DNA extraction protocol from tilapia fin tissue that enables the use of 96-well plates using a fluid micro-dispenser robot, thus effectively decreasing the time, cost and handling error of the DNA preparation stage. The method is based on the DNA salting out procedure and all fluid transfers are designed for use with a micro dispenser robot. The criterion of success was defined as extraction of at least 2 μg of DNA, which enables at least 100 PCR reactions. A total of 3163 samples met this criterion (98%). The cost of the proposed in-house protocol is approximately ten-fold lower than the commercial alternatives. The suggested high-throughput extraction protocol may be adjusted by slight modifications to handle other tissues. © 2005 Elsevier B.V. All rights reserved.
Scientific Publication
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