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Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression
Year:
2002
Source of publication :
Phytopathology
Authors :
Elad, Yigal
;
.
Fischer, Erwin
;
.
Guetsky, Ruth
;
.
Shtienberg, Dan
;
.
Volume :
92
Co-Authors:
Guetsky, R., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shtienberg, D., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Fischer, E., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
976
To page:
985
(
Total pages:
10
)
Abstract:
Two biocontrol agents, a yeast (Pichia guilermondii) and a bacterium (Bacillus mycoides), were tested separately and together for suppression of Botrytis cinerea on strawberry leaves and plants. Scanning electron microscopy revealed significant inhibition of Botrytis cinerea conidial germination in the presence of Pichia guilermondii, whereas Bacillus mycoides caused breakage and destruction of conidia. When both biocontrol agents were applied in a mixture, conidial destruction was more severe. The modes of action of each of the biocontrol agents were elucidated and the relative quantitative contribution of each mechanism to suppression of Botrytis cinerea was estimated using multiple regression with dummy variables. The improvement in control efficacy achieved by introducing one or more mechanisms at a time was calculated. Pichia guilermondii competed with Botrytis cinerea for glucose, sucrose, adenine, histidine, and folic acid. Viability of the yeast cells played a crucial role in suppression of Botrytis cinerea and they secreted an inhibitory compound that had an acropetal effect and was not volatile. Bacillus mycoides did not compete for any of the sugars, amino acids, or vitamins examined at a level that would affect Botrytis cinerea development. Viable cells and the compounds secreted by them contributed similarly to Botrytis cinerea suppression. The bacteria secreted volatile and non-volatile inhibitory compounds and activated the defense systems of the host. The nonvolatile compounds had both acropetal and basipetal effects. Mixture of Pichia guilermondii and Bacillus mycoides resulted in additive activity compared with their separate application. The combined activity was due to the summation of biocontrol mechanisms of both agents. This work provides a theoretical explanation for our previous findings of reduced disease control variability with a mixture of Pichia guilermondii and Bacillus mycoides.
Note:
Related Files :
biological control agent
cell secretion
competitive ability
folic acid
multiple regression
Plant Disease
sucrose
Yeast
Show More
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21461
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
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Scientific Publication
Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression
92
Guetsky, R., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Shtienberg, D., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Fischer, E., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression
Two biocontrol agents, a yeast (Pichia guilermondii) and a bacterium (Bacillus mycoides), were tested separately and together for suppression of Botrytis cinerea on strawberry leaves and plants. Scanning electron microscopy revealed significant inhibition of Botrytis cinerea conidial germination in the presence of Pichia guilermondii, whereas Bacillus mycoides caused breakage and destruction of conidia. When both biocontrol agents were applied in a mixture, conidial destruction was more severe. The modes of action of each of the biocontrol agents were elucidated and the relative quantitative contribution of each mechanism to suppression of Botrytis cinerea was estimated using multiple regression with dummy variables. The improvement in control efficacy achieved by introducing one or more mechanisms at a time was calculated. Pichia guilermondii competed with Botrytis cinerea for glucose, sucrose, adenine, histidine, and folic acid. Viability of the yeast cells played a crucial role in suppression of Botrytis cinerea and they secreted an inhibitory compound that had an acropetal effect and was not volatile. Bacillus mycoides did not compete for any of the sugars, amino acids, or vitamins examined at a level that would affect Botrytis cinerea development. Viable cells and the compounds secreted by them contributed similarly to Botrytis cinerea suppression. The bacteria secreted volatile and non-volatile inhibitory compounds and activated the defense systems of the host. The nonvolatile compounds had both acropetal and basipetal effects. Mixture of Pichia guilermondii and Bacillus mycoides resulted in additive activity compared with their separate application. The combined activity was due to the summation of biocontrol mechanisms of both agents. This work provides a theoretical explanation for our previous findings of reduced disease control variability with a mixture of Pichia guilermondii and Bacillus mycoides.
Scientific Publication
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