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Senescence-induced RNases in tomato
Year:
1998
Source of publication :
Plant Molecular Biology
Authors :
Burd, Shaul
;
.
Kaltchiski, Andrei
;
.
Lers, Amnon
;
.
Lomaniec, Ella
;
.
Volume :
36
Co-Authors:
Lers, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Khalchitski, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Green, P.J., MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, United States
Facilitators :
From page:
439
To page:
449
(
Total pages:
11
)
Abstract:
A main feature of leaf senescence is the hydrolysis of macromolecules by hydrolases of various types, and redistribution of released materials. We have initiated a study for the characterization of RNases involved in nucleic acid catabolism during senescence. Using a PCR-based cloning approach we isolated from tomato two senescence-induced RNase cDNA clones. Each of these cDNAs hybridized to a senescence-induced transcript in northern analysis. One RNase cDNA was identical to the tomato LX RNase while the second corresponded to the LE RNase. Both LX and LE RNase genes had originally been demonstrated to be induced after phosphate starvation of tomato cell culture but nothing was known about their expression or function in plants. We observed that the expression of the LX and LE genes is induced in leaves during an advanced stage of senescence with the LX transcript level being much more induced than that of LE. Low-level expression of the RNase genes was observed in flowers and artificially senescing detached leaves while no expression could be detected in stems, roots, or fruits at different ripening stages. Ethylene activated the LX gene expression in detached young leaves while LE gene expression, which could be transiently induced by wounding, appeared to be activated by abscisic acid. We suggest that the LX RNase has a role in RNA catabolism in the final stage of senescence, and LE may function during wounding as a plant defense protein.
Note:
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More details
DOI :
10.1023/A:1005993024161
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21462
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
Senescence-induced RNases in tomato
36
Lers, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Khalchitski, A., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Produce, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Green, P.J., MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, United States
Senescence-induced RNases in tomato
A main feature of leaf senescence is the hydrolysis of macromolecules by hydrolases of various types, and redistribution of released materials. We have initiated a study for the characterization of RNases involved in nucleic acid catabolism during senescence. Using a PCR-based cloning approach we isolated from tomato two senescence-induced RNase cDNA clones. Each of these cDNAs hybridized to a senescence-induced transcript in northern analysis. One RNase cDNA was identical to the tomato LX RNase while the second corresponded to the LE RNase. Both LX and LE RNase genes had originally been demonstrated to be induced after phosphate starvation of tomato cell culture but nothing was known about their expression or function in plants. We observed that the expression of the LX and LE genes is induced in leaves during an advanced stage of senescence with the LX transcript level being much more induced than that of LE. Low-level expression of the RNase genes was observed in flowers and artificially senescing detached leaves while no expression could be detected in stems, roots, or fruits at different ripening stages. Ethylene activated the LX gene expression in detached young leaves while LE gene expression, which could be transiently induced by wounding, appeared to be activated by abscisic acid. We suggest that the LX RNase has a role in RNA catabolism in the final stage of senescence, and LE may function during wounding as a plant defense protein.
Scientific Publication
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