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אסיף מאגר המחקר החקלאי
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Characterization of the auxin synthesis genes of Erwinia herbicola pv. gypsophilae
Year:
1997
Source of publication :
Israel Journal of Plant Sciences
Authors :
Gafni, Yedidya
;
.
Kunik, T.
;
.
Lichter, Amnon
;
.
Manulis-Sasson, Shulamit
;
.
Ophir, Yakir
;
.
Volume :
45
Co-Authors:
Gafni, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Kunik, T., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lichter, A., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Ophir, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Department of Biotechnology, Israel Inst. for Biological Research, P.O. Box 19, Ness Ziona 74100, Israel
Facilitators :
From page:
279
To page:
284
(
Total pages:
6
)
Abstract:
We present the DNA sequence of some 3590 base pairs from the virulence plasmid of the crown-gall-forming bacterium Erwinia herbicola pv. gypsophilae strain PD713. This region includes the entire transcription unit of an operon encompassing the IAA synthesis genes. These genes, designated iaaM and iaaH, reside on a 78-MDa native plasmid. The sequence analysis indicates that the operon contains two open reading frames of 562 (iaaM) and 460 amino acids (iaaH), corresponding to proteins with molecular weight of 62,473 and 49,300 daltons, respectively. When cloned and expressed in minicell-producing E. coli, this sequence encodes production of two proteins, 49 and 62 kDa. Both genes show moderate to high homology to IAA synthesis genes from other plant-associated bacteria. Analysis by primer extension of the transcription initiation site located it 63 bases upstream of the translation initiation codon.
Note:
Related Files :
biosynthesis
Open reading frame
Plasmid
sequence analysis
sequence homology
transcription initiation
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21475
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
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Characterization of the auxin synthesis genes of Erwinia herbicola pv. gypsophilae
45
Gafni, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Kunik, T., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lichter, A., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Ophir, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Department of Biotechnology, Israel Inst. for Biological Research, P.O. Box 19, Ness Ziona 74100, Israel
Characterization of the auxin synthesis genes of Erwinia herbicola pv. gypsophilae
We present the DNA sequence of some 3590 base pairs from the virulence plasmid of the crown-gall-forming bacterium Erwinia herbicola pv. gypsophilae strain PD713. This region includes the entire transcription unit of an operon encompassing the IAA synthesis genes. These genes, designated iaaM and iaaH, reside on a 78-MDa native plasmid. The sequence analysis indicates that the operon contains two open reading frames of 562 (iaaM) and 460 amino acids (iaaH), corresponding to proteins with molecular weight of 62,473 and 49,300 daltons, respectively. When cloned and expressed in minicell-producing E. coli, this sequence encodes production of two proteins, 49 and 62 kDa. Both genes show moderate to high homology to IAA synthesis genes from other plant-associated bacteria. Analysis by primer extension of the transcription initiation site located it 63 bases upstream of the translation initiation codon.
Scientific Publication
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