נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Enzymic maceration of citrus callus and the regeneration of plants from single cells
Year:
1975
Source of publication :
Journal of Experimental Botany
Authors :
Button, James
;
.
Volume :
26
Co-Authors:
Button, J., Plant Tissue Culture Research Unit, University of Natal, Pietermaritzburg, South Africa
Botha, C.E.J., Plant Tissue Culture Research Unit, University of Natal, Pietermaritzburg, South Africa
Facilitators :
From page:
723
To page:
729
(
Total pages:
7
)
Abstract:
The maceration medium comprised a basal nutrient medium (BM) containing an optimum concentration of 3% (w/v) sucrose. Mannitol and sorbitol were inferior osmotica. Addition of potassium dextran sulphate adversely affected maceration. 'Macerozyme' was not as effective as 'Macerase' in the production of single cells. The optimal concentration of 'Macerase' was found to be 2-3% (w/v).Single cells obtained by filtering the macerate were rinsed with BM and cultured in, and on, agar media comprising: BM; BM + 500 mg 1 -1 malt extract (ME); and BM + 10% (v/v) coconut milk (CM). No growth or organization was observed in cultures where cells were mixed in with warm medium prior to gelling. When spread on the surface of gelled media supplemented with ME and CM, proliferation and organization occurred. Many microscopic globular proembryoids developed within 3 weeks on the supplemented media. Microscopic torpedo-shaped embryoids were frequently observed on BM + CM, rarely on BM + ME, and not at all on unsupplemented BM.The high frequency of microscopic globular proembryoids, and later of macroscopic pseudo-bulbils, formed on BM + ME leads us to postulate that pseudobulbils are derived from globular proembryoids in which polarity is not established by the 16 to 32-cell stage. Microscopic torpedo-shaped embryoids probably give rise to macroscopic heart-shaped embryoids which develop into plantlets.The technique reported in this article provides an ideal system for examining embryogenesis per se and for studying the effects of various treatments on embryogenesis and organ differentiation in vitro. It also affords excellent opportunities for the breeding of solid mutant plants. © 1975 Oxford University Press.
Note:
Related Files :
Show More
Related Content
More details
DOI :
10.1093/jxb/26.5.723
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21499
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
Enzymic maceration of citrus callus and the regeneration of plants from single cells
26
Button, J., Plant Tissue Culture Research Unit, University of Natal, Pietermaritzburg, South Africa
Botha, C.E.J., Plant Tissue Culture Research Unit, University of Natal, Pietermaritzburg, South Africa
Enzymic maceration of citrus callus and the regeneration of plants from single cells
The maceration medium comprised a basal nutrient medium (BM) containing an optimum concentration of 3% (w/v) sucrose. Mannitol and sorbitol were inferior osmotica. Addition of potassium dextran sulphate adversely affected maceration. 'Macerozyme' was not as effective as 'Macerase' in the production of single cells. The optimal concentration of 'Macerase' was found to be 2-3% (w/v).Single cells obtained by filtering the macerate were rinsed with BM and cultured in, and on, agar media comprising: BM; BM + 500 mg 1 -1 malt extract (ME); and BM + 10% (v/v) coconut milk (CM). No growth or organization was observed in cultures where cells were mixed in with warm medium prior to gelling. When spread on the surface of gelled media supplemented with ME and CM, proliferation and organization occurred. Many microscopic globular proembryoids developed within 3 weeks on the supplemented media. Microscopic torpedo-shaped embryoids were frequently observed on BM + CM, rarely on BM + ME, and not at all on unsupplemented BM.The high frequency of microscopic globular proembryoids, and later of macroscopic pseudo-bulbils, formed on BM + ME leads us to postulate that pseudobulbils are derived from globular proembryoids in which polarity is not established by the 16 to 32-cell stage. Microscopic torpedo-shaped embryoids probably give rise to macroscopic heart-shaped embryoids which develop into plantlets.The technique reported in this article provides an ideal system for examining embryogenesis per se and for studying the effects of various treatments on embryogenesis and organ differentiation in vitro. It also affords excellent opportunities for the breeding of solid mutant plants. © 1975 Oxford University Press.
Scientific Publication
You may also be interested in