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Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato
Year:
2006
Source of publication :
Planta
Authors :
Aussenberg, Elitsur R.
;
.
Gal, Tali Z.
;
.
Kapulnik, Yoram
;
.
Koltai, Hinanit
;
.
Volume :
224
Co-Authors:
Gal, T.Z., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel
Aussenberg, E.R., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, 76100 Rehovot, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, ARO, Volcani Center, 50250 Bet Dagan, Israel
Koltai, H., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel, Department of Ornamental Horticulture, ARO, Volcani Center, 50250 Bet Dagan, Israel
Facilitators :
From page:
155
To page:
162
(
Total pages:
8
)
Abstract:
A group of plant proteins, expansins, have been identified as wall-loosening factors and as facilitators of cell expansion in vivo. The root knot nematode Meloidogyne javanica establishes a permanent feeding site composed of giant cells surrounded by gall tissue. We used quantitative PCR and in situ localization to demonstrate the induction of a tomato (Lycopersicon esculentum cv. VF36) expansin (LeEXPA5) expression in gall cells adjacent to the nematode feeding cells. To further characterize the biological role of LeEXPA5 we have generated LeEXPA5-antisense transgenic roots. The ability of the nematode to establish a feeding site and complete its life cycle, the average root cell size and the rate of root elongation were determined for the transgenic roots, as well as the level of LeEXPA5 expression in non-infected and nematode-infected roots. Our results demonstrated that a decrease of LeEXPA5 expression reduces the ability of the nematode to complete its life cycle in transgenic roots. We suggest that a plant-originated expansin is necessary for a successful parasitic nematode-plant interaction. © Springer-Verlag 2006.
Note:
Related Files :
Animal
Animals
cell enlargement
gall
Genetics
metabolism
nematode
Parasite
Parasitology
transgenic plant
Show More
Related Content
More details
DOI :
10.1007/s00425-005-0204-x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21517
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
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Scientific Publication
Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato
224
Gal, T.Z., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel
Aussenberg, E.R., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, 76100 Rehovot, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, ARO, Volcani Center, 50250 Bet Dagan, Israel
Koltai, H., Department of Genomics, ARO, Volcani Center, 50250 Bet Dagan, Israel, Department of Ornamental Horticulture, ARO, Volcani Center, 50250 Bet Dagan, Israel
Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato
A group of plant proteins, expansins, have been identified as wall-loosening factors and as facilitators of cell expansion in vivo. The root knot nematode Meloidogyne javanica establishes a permanent feeding site composed of giant cells surrounded by gall tissue. We used quantitative PCR and in situ localization to demonstrate the induction of a tomato (Lycopersicon esculentum cv. VF36) expansin (LeEXPA5) expression in gall cells adjacent to the nematode feeding cells. To further characterize the biological role of LeEXPA5 we have generated LeEXPA5-antisense transgenic roots. The ability of the nematode to establish a feeding site and complete its life cycle, the average root cell size and the rate of root elongation were determined for the transgenic roots, as well as the level of LeEXPA5 expression in non-infected and nematode-infected roots. Our results demonstrated that a decrease of LeEXPA5 expression reduces the ability of the nematode to complete its life cycle in transgenic roots. We suggest that a plant-originated expansin is necessary for a successful parasitic nematode-plant interaction. © Springer-Verlag 2006.
Scientific Publication
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