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Improved 2,4-Dinitrophenvlhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid
Year:
1974
Authors :
Navon, Amos
;
.
Volume :
29
Co-Authors:
Navon, A., Division of Entomology, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, Israel
Levinson, H.Z., Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany
Facilitators :
From page:
777
To page:
780
(
Total pages:
4
)
Abstract:
Microamounts of vitamin C could be readily determined in 20 μ1-samples using the 2.4-dinitrophenylhydrazine method together with separation by thin layer chromatography. The condensation reaction was carried out for 5 min at 100 °C on a glass fibre disc. Purification of vitamin C hydrazones was accomplished by repeated separation on TLC plates. An aqueous solution of 65% acetic acid was employed to dissolve the vitamin C hydrazones, providing maximal absorbance at 500 nm. The minimum amount detectable by this method is 0.4 μg of dehydroascorbic acid. The macrodetermination of vitamin C was improved by simplifying a previous method and employing 65% aqueous acetic acid as a solvent for the hydrazones. © 1974, Walter de Gruyter. All rights reserved.
Note:
Related Files :
2,4-Dinitrophenylhydrazine
2,4 dinitrophenylhydrazine
2,4 dinitrophenylhydrazone derivative
Phenylhydrazines
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More details
DOI :
10.1515/znc-1974-11-1222
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Note
;
.
Language:
English
Editors' remarks:
ID:
21585
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
Scientific Publication
Improved 2,4-Dinitrophenvlhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid
29
Navon, A., Division of Entomology, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, Israel
Levinson, H.Z., Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany
Improved 2,4-Dinitrophenvlhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid
Microamounts of vitamin C could be readily determined in 20 μ1-samples using the 2.4-dinitrophenylhydrazine method together with separation by thin layer chromatography. The condensation reaction was carried out for 5 min at 100 °C on a glass fibre disc. Purification of vitamin C hydrazones was accomplished by repeated separation on TLC plates. An aqueous solution of 65% acetic acid was employed to dissolve the vitamin C hydrazones, providing maximal absorbance at 500 nm. The minimum amount detectable by this method is 0.4 μg of dehydroascorbic acid. The macrodetermination of vitamin C was improved by simplifying a previous method and employing 65% aqueous acetic acid as a solvent for the hydrazones. © 1974, Walter de Gruyter. All rights reserved.
Scientific Publication
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