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אסיף מאגר המחקר החקלאי
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Trait stability and fitness of the heat tolerant entomopathogenic nematode Heterorhabditis bacteriophora IS5 strain
Year:
1996
Source of publication :
biological control (source)
Authors :
Glazer, Itamar
;
.
Shapiro, David I.
;
.
Volume :
6
Co-Authors:
Shapiro, D.I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Segal, D., Dept. of Molec. Microbiol./Biotech., Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
238
To page:
244
(
Total pages:
7
)
Abstract:
Natural populations of entomopathogenic nematodes may possess novel traits that offer advantages in biological control. If, however, the populations cannot be reared under laboratory conditions, and if their beneficial traits are unstable, then increases in biocontrol efficacy will be minimal. The stability of heat tolerance in, and fitness under laboratory conditions of, a newly discovered strain of Heterorhabditis bacteriophora (designated IS5) were investigated. Trait stability and fitness assays were conducted after multiple passages through Galleria mellonella. Assays were conducted on IS5 populations reared at 30 and 25°C. Trait stability was determined as survival at elevated temperatures. Relative to the commercially available strain of H. bacteriophora (HP88), the IS5 strain exhibited greater heat tolerance after 12 passages regardless of selection pressure. The population of IS5 reared at 30°C exhibited greater heat tolerance than the population reared at 25°C. Results indicated that the heat tolerance trait is genetically based. Estimates of fitness were based on virulence, infectivity, reproductive potential, and storage capacity. The virulence, infectivity, and reproductive potential of IS5 nematodes were greater than or equal to that of HP88 nematodes. The heat tolerance trait in IS5 was retained after 6 weeks of storage. The IS5 strain survived significantly longer at 25°C than at 10°C, indicating that these nematodes may be cold sensitive. The results of this paper will expedite future genetic, biochemical, and field efficacy studies on H. bacteriophora IS5.
Note:
Related Files :
biological control
fitness
heat tolerance
Heterorhabditidae
Heterorhabditis bacteriophora
trait stability
Show More
Related Content
More details
DOI :
10.1006/bcon.1996.0030
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:

Itamar's own PDF.

ID:
21611
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
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Scientific Publication
Trait stability and fitness of the heat tolerant entomopathogenic nematode Heterorhabditis bacteriophora IS5 strain
6
Shapiro, D.I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Segal, D., Dept. of Molec. Microbiol./Biotech., Tel Aviv University, Tel Aviv 69978, Israel
Trait stability and fitness of the heat tolerant entomopathogenic nematode Heterorhabditis bacteriophora IS5 strain
Natural populations of entomopathogenic nematodes may possess novel traits that offer advantages in biological control. If, however, the populations cannot be reared under laboratory conditions, and if their beneficial traits are unstable, then increases in biocontrol efficacy will be minimal. The stability of heat tolerance in, and fitness under laboratory conditions of, a newly discovered strain of Heterorhabditis bacteriophora (designated IS5) were investigated. Trait stability and fitness assays were conducted after multiple passages through Galleria mellonella. Assays were conducted on IS5 populations reared at 30 and 25°C. Trait stability was determined as survival at elevated temperatures. Relative to the commercially available strain of H. bacteriophora (HP88), the IS5 strain exhibited greater heat tolerance after 12 passages regardless of selection pressure. The population of IS5 reared at 30°C exhibited greater heat tolerance than the population reared at 25°C. Results indicated that the heat tolerance trait is genetically based. Estimates of fitness were based on virulence, infectivity, reproductive potential, and storage capacity. The virulence, infectivity, and reproductive potential of IS5 nematodes were greater than or equal to that of HP88 nematodes. The heat tolerance trait in IS5 was retained after 6 weeks of storage. The IS5 strain survived significantly longer at 25°C than at 10°C, indicating that these nematodes may be cold sensitive. The results of this paper will expedite future genetic, biochemical, and field efficacy studies on H. bacteriophora IS5.
Scientific Publication
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