נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Detection, quantification and characterization of Erwinia carotovora ssp. carotovora contaminating pepper seeds
Year:
2001
Source of publication :
Plant Pathology
Authors :
Gefen, Tanya
;
.
Hadas, Rivka
;
.
Kritzman, Giora
;
.
Volume :
50
Co-Authors:
Hadas, R., Official Seed Testing Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kritzman, G., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gefen, T., Official Seed Testing Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
117
To page:
123
(
Total pages:
7
)
Abstract:
Erwinia carotovora ssp. carotovora (Ecc) was isolated from commercial pepper (Capsicum annuum) seed lots and identified according to biochemical and pathogenicity tests, cellular fatty acid composition, and PCR reaction with primers based on the pel gene sequence. RAPD-PCR analysis using nine arbitrary primers showed similarity between Ecc isolates obtained from pepper. Ecc isolates from pepper, tomato, potato and cabbage formed four distinct groups, according to the original host. A natural Ecc population higher than 100 CFU g-1 seed significantly affected seed germination. A significant correlation between the level of Ecc on the seeds and the percentage of diseased seedlings was also observed. Surface sterilization of the seeds with sodium hypochlorite eliminated the pathogen, indicating that the bacteria were located on the surface of the seeds. Contaminated pepper seeds can be a primary and important source of inoculum.
Note:
Related Files :
bacterium detection
bacterium identification
Capsicum annuum
disease incidence
Seed
Show More
Related Content
More details
DOI :
10.1046/j.1365-3059.2001.00540.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21660
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
Scientific Publication
Detection, quantification and characterization of Erwinia carotovora ssp. carotovora contaminating pepper seeds
50
Hadas, R., Official Seed Testing Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kritzman, G., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gefen, T., Official Seed Testing Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Detection, quantification and characterization of Erwinia carotovora ssp. carotovora contaminating pepper seeds
Erwinia carotovora ssp. carotovora (Ecc) was isolated from commercial pepper (Capsicum annuum) seed lots and identified according to biochemical and pathogenicity tests, cellular fatty acid composition, and PCR reaction with primers based on the pel gene sequence. RAPD-PCR analysis using nine arbitrary primers showed similarity between Ecc isolates obtained from pepper. Ecc isolates from pepper, tomato, potato and cabbage formed four distinct groups, according to the original host. A natural Ecc population higher than 100 CFU g-1 seed significantly affected seed germination. A significant correlation between the level of Ecc on the seeds and the percentage of diseased seedlings was also observed. Surface sterilization of the seeds with sodium hypochlorite eliminated the pathogen, indicating that the bacteria were located on the surface of the seeds. Contaminated pepper seeds can be a primary and important source of inoculum.
Scientific Publication
You may also be interested in