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Quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells
Year:
1994
Source of publication :
The Journal of Membrane Biology
Authors :
Ben Hayyim, Gosal
;
.
Yermiyahu, Uri
;
.
Volume :
138
Co-Authors:
Yermiyahu, U., Department of Field and Vegetable Crops, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Nir, S., Seagram Center for Soil and Water Sciences, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Ben-Hayyim, G., Institute of Horticulture, The Volcani Center, Bet Dagan, 50250, Israel
Kafkafi, U., Department of Field and Vegetable Crops, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Facilitators :
From page:
55
To page:
63
(
Total pages:
9
)
Abstract:
The presence of Ca2+ ions in solution is vital for root growth. The plasma membrane is one of the first sites where competition between Ca2+ and other ions occurs. We studied the competition between Ca2+ and Na+ or Mg2+ for sorption sites on the plasma membrane of melon root cells. Sorption of 45Ca2+ to right-side-out PM vesicles of melon (Cucumis melo L.) roots (prepared by aqueous two-phase partitioning) was studied at various Ca2+ concentrations, in the presence of increasing concentrations of Na+ or Mg2+ chlorides. Experimentally determined amounts of Ca2+ sorbed to the plasma membrane vesicles agreed fairly well with those calculated from a competitive sorption model. The best fit of the model to the experimental data was obtained for an average surface area of 370 Å2 per charge, and binding coefficients for Na+, Mg2+ and Ca2+ of 0.8, 9 and 50 m-1, respectively. Our results suggest that nonphospholipid components in the plasma membrane contribute significantly to Ca2+ binding. The high affinity of Ca2+ binding to the plasma membrane found in this study might explain the specific role of Ca2+ in relieving salt stress in plant roots. © 1994 Springer-Verlag New York Inc.
Note:
Related Files :
Binding, Competitive
Binding Sites
calcium
cell membrane
Magnesium
Models, Biological
plant root
Plants
sodium
Show More
Related Content
More details
DOI :
10.1007/BF00211069
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21670
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
Scientific Publication
Quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells
138
Yermiyahu, U., Department of Field and Vegetable Crops, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Nir, S., Seagram Center for Soil and Water Sciences, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Ben-Hayyim, G., Institute of Horticulture, The Volcani Center, Bet Dagan, 50250, Israel
Kafkafi, U., Department of Field and Vegetable Crops, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot, 76-100, Israel
Quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells
The presence of Ca2+ ions in solution is vital for root growth. The plasma membrane is one of the first sites where competition between Ca2+ and other ions occurs. We studied the competition between Ca2+ and Na+ or Mg2+ for sorption sites on the plasma membrane of melon root cells. Sorption of 45Ca2+ to right-side-out PM vesicles of melon (Cucumis melo L.) roots (prepared by aqueous two-phase partitioning) was studied at various Ca2+ concentrations, in the presence of increasing concentrations of Na+ or Mg2+ chlorides. Experimentally determined amounts of Ca2+ sorbed to the plasma membrane vesicles agreed fairly well with those calculated from a competitive sorption model. The best fit of the model to the experimental data was obtained for an average surface area of 370 Å2 per charge, and binding coefficients for Na+, Mg2+ and Ca2+ of 0.8, 9 and 50 m-1, respectively. Our results suggest that nonphospholipid components in the plasma membrane contribute significantly to Ca2+ binding. The high affinity of Ca2+ binding to the plasma membrane found in this study might explain the specific role of Ca2+ in relieving salt stress in plant roots. © 1994 Springer-Verlag New York Inc.
Scientific Publication
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