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Partial sequence and expression of the gene for and activity of the sodium glucose transporter in the small intestine of fed, starved and refed chickens
Year:
2000
Source of publication :
Journal of Nutrition
Authors :
Mabjeesh, Sameer Jermaya
;
.
Volume :
130
Co-Authors:
Gal-Garber, O., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mabjeesh, S.J., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sklan, D., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Uni, Z., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
2174
To page:
2179
(
Total pages:
6
)
Abstract:
A 970 bp cDNA Na+/glucose cotransporter (SGLT1) was isolated and sequenced from chicken jejunum by reverse transcriptase polymerase chain reaction (RT-PCR) using primers based on conserved regions. Using the 970 bp PCR product as a specific probe, Northern Blot hybridization indicated a transcript of ca. 4 kb. The isolated chicken intestinal SGLT1 cDNA was used to quantitate mRNA expression. Glucose uptake activity and kinetics were determined in brush border membrane vesicles (BBMV) from jejunum tissue of chickens which were either fed, food-deprived or refed following food deprivation. Net glucose uptake to BBMV was higher (P < 0.02) in the control and refed chicks (149 ± 11.9, 139.6 ± 7.43 pmol · mg protein-1 · s-1) than in food-deprived chicks (107 ± 4.23 pmol · mg protein-1 · s-1). The k(m) (150 μmol/L) and Vmax (1111.1 pmol · mg protein-1 · s-1) were higher in the food-deprived chicks compared to control and refed birds (25, 24 μmol/L and 227, 142 pmol · mg protein-1 · s-1, respectively). Expression of SGLT1 mRNA was significantly enhanced in the food-deprived and refed birds. In food-deprived chicks the lower affinity and higher activity of the SGLT1 transporter for glucose were accompanied by higher expression of mRNA which might indicate that the transporter was upregulated by low substrate concentration. Quantification of expression of intestinal mRNA of SGLT1 provides important information concerning control of nutrient uptake.
Note:
Related Files :
animal experiment
Animals
animal tissue
gene expression
Glucose transporter
Male
nutrition
sheep
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21709
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
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Scientific Publication
Partial sequence and expression of the gene for and activity of the sodium glucose transporter in the small intestine of fed, starved and refed chickens
130
Gal-Garber, O., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mabjeesh, S.J., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sklan, D., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Uni, Z., Faculty of Agricultural, Food and Envtl. Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Partial sequence and expression of the gene for and activity of the sodium glucose transporter in the small intestine of fed, starved and refed chickens
A 970 bp cDNA Na+/glucose cotransporter (SGLT1) was isolated and sequenced from chicken jejunum by reverse transcriptase polymerase chain reaction (RT-PCR) using primers based on conserved regions. Using the 970 bp PCR product as a specific probe, Northern Blot hybridization indicated a transcript of ca. 4 kb. The isolated chicken intestinal SGLT1 cDNA was used to quantitate mRNA expression. Glucose uptake activity and kinetics were determined in brush border membrane vesicles (BBMV) from jejunum tissue of chickens which were either fed, food-deprived or refed following food deprivation. Net glucose uptake to BBMV was higher (P < 0.02) in the control and refed chicks (149 ± 11.9, 139.6 ± 7.43 pmol · mg protein-1 · s-1) than in food-deprived chicks (107 ± 4.23 pmol · mg protein-1 · s-1). The k(m) (150 μmol/L) and Vmax (1111.1 pmol · mg protein-1 · s-1) were higher in the food-deprived chicks compared to control and refed birds (25, 24 μmol/L and 227, 142 pmol · mg protein-1 · s-1, respectively). Expression of SGLT1 mRNA was significantly enhanced in the food-deprived and refed birds. In food-deprived chicks the lower affinity and higher activity of the SGLT1 transporter for glucose were accompanied by higher expression of mRNA which might indicate that the transporter was upregulated by low substrate concentration. Quantification of expression of intestinal mRNA of SGLT1 provides important information concerning control of nutrient uptake.
Scientific Publication
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