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אסיף מאגר המחקר החקלאי
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Induction of serotonin release from mast cells by lymphocyte activators is dependent upon implantation of lymphocyte plasma membrane components
Year:
1991
Source of publication :
Experimental Cell Research
Authors :
Lapidot, Moshe
;
.
Volume :
194
Co-Authors:
Abramovitz, B.
Altboum, I.
Lapidot, M., Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel, Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
Loyter, A., Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
Zan-Bar, I.
Facilitators :
From page:
228
To page:
231
(
Total pages:
4
)
Abstract:
Plasma membranes (PM) isolated from mouse splenic lymphocytes were successfully fused to rat basophilic leukemia (RBL) cells using intact Sendai virus virions (SV). A two-step procedure was used in which SV were first fused with the PM to create PM + SV vesicles; the vesicles formed were then incubated with the RBL cells. Insertion of lymphocyte PM into the RBL cell's membrane endow a high rate of serotonin secretion upon stimulation of the implanted RBL cells with antimouse Ig antibodies or with concanavalin-A. The results of the present work clearly suggest that activation signals can be delivered via implantation of foreign membrane preparation containing specific receptors, thus rendering the target cells susceptible to stimulation by specific reagents. © 1991.
Note:
Related Files :
Animal
animal cell
cell membrane
Kinetics
Leukemia, Experimental
membrane fusion
mice
mouse
serotonin release
Show More
Related Content
More details
DOI :
10.1016/0014-4827(91)90358-2
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21711
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
Scientific Publication
Induction of serotonin release from mast cells by lymphocyte activators is dependent upon implantation of lymphocyte plasma membrane components
194
Abramovitz, B.
Altboum, I.
Lapidot, M., Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel, Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
Loyter, A., Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
Zan-Bar, I.
Induction of serotonin release from mast cells by lymphocyte activators is dependent upon implantation of lymphocyte plasma membrane components
Plasma membranes (PM) isolated from mouse splenic lymphocytes were successfully fused to rat basophilic leukemia (RBL) cells using intact Sendai virus virions (SV). A two-step procedure was used in which SV were first fused with the PM to create PM + SV vesicles; the vesicles formed were then incubated with the RBL cells. Insertion of lymphocyte PM into the RBL cell's membrane endow a high rate of serotonin secretion upon stimulation of the implanted RBL cells with antimouse Ig antibodies or with concanavalin-A. The results of the present work clearly suggest that activation signals can be delivered via implantation of foreign membrane preparation containing specific receptors, thus rendering the target cells susceptible to stimulation by specific reagents. © 1991.
Scientific Publication
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