נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
The GHOST terminal oxidase regulates developmental programming in tomato fruit
Year:
2004
Source of publication :
Plant, Cell and Environment
Authors :
Chen, Lea
;
.
Volume :
27
Co-Authors:
Barr, J., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States
White, W.S., Dept. of Food Sci. and Hum. Nutr., Iowa State University, Ames, IA 50011, United States
Chen, L., Dept. of Food Sci. and Hum. Nutr., Iowa State University, Ames, IA 50011, United States
Bae, H., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States
Rodermel, S., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States, Dept. Genet., Devmt. and Cell Biol., 353 Bessey Hall, Iowa State University, Ames, IA 50011, United States
Facilitators :
From page:
840
To page:
852
(
Total pages:
13
)
Abstract:
The tomato ghost and Arabidopsis immutans variegation mutants define orthologous genes for a chloroplast quinol oxidase that bears similarity to the mitochondrial alternative oxidase. In this study tomato fruit ripening was used as a model to explore the function of this oxidase. It was found that a lack of GH impacts the biogenesis of chloroplasts and chromoplasts, as well as pericarp tissue morphogenesis, during the ripening process. Most ripening-related genes are expressed normally during gh fruit development, but late in the process there is a specific repression of mRNA accumulation from carotenoid biosynthetic genes, perhaps due to retrograde (plastid-to-nucleus) signalling pathways mediated by lycopene. Metabolic profiling revealed that wild-type red-ripe fruit accumulate multiple isomers of phytoene, phytofluene, and ζ-carotene, but that the composition of these isomers is different in ripe gh fruit. Profiling also revealed that there is an enhanced ratio of β-carotene to lycopene in the ripe gh fruit. This explains the orange colour of these fruit, and is consistent with the proposal that the β-cyclization step plays a pivotal role in regulating the partitioning of pathway intermediates. The total carotenoid content of the mutant fruit is significantly higher than normal due to an accumulation of phytoene. Phytoene accumulation, together with an up-regulation of GH transcripts during ripening, supports the notion that GH plays a central role in carotenogenesis in chromoplasts. However, GH mRNAs are also expressed in tissues and organs other than fruit. This is consistent with the possibility that GH mediates quinol oxidation from a variety of pathways that converge on plastoquinol in plastid membranes.
Note:
Related Files :
arabidopsis
chloroplast
Lycopersicon esculentum
metabolic profiling
Phytoene desaturase
Retrograde signalling
tomato
Show More
Related Content
More details
DOI :
10.1111/j.1365-3040.2004.01190.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21729
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
Scientific Publication
The GHOST terminal oxidase regulates developmental programming in tomato fruit
27
Barr, J., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States
White, W.S., Dept. of Food Sci. and Hum. Nutr., Iowa State University, Ames, IA 50011, United States
Chen, L., Dept. of Food Sci. and Hum. Nutr., Iowa State University, Ames, IA 50011, United States
Bae, H., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States
Rodermel, S., Dept. Genet., Devmt. and Cell Biol., Iowa State University, Ames, IA 50011, United States, Interdepartmental Genetics Major, Iowa State University, Ames, IA 50011, United States, Dept. Genet., Devmt. and Cell Biol., 353 Bessey Hall, Iowa State University, Ames, IA 50011, United States
The GHOST terminal oxidase regulates developmental programming in tomato fruit
The tomato ghost and Arabidopsis immutans variegation mutants define orthologous genes for a chloroplast quinol oxidase that bears similarity to the mitochondrial alternative oxidase. In this study tomato fruit ripening was used as a model to explore the function of this oxidase. It was found that a lack of GH impacts the biogenesis of chloroplasts and chromoplasts, as well as pericarp tissue morphogenesis, during the ripening process. Most ripening-related genes are expressed normally during gh fruit development, but late in the process there is a specific repression of mRNA accumulation from carotenoid biosynthetic genes, perhaps due to retrograde (plastid-to-nucleus) signalling pathways mediated by lycopene. Metabolic profiling revealed that wild-type red-ripe fruit accumulate multiple isomers of phytoene, phytofluene, and ζ-carotene, but that the composition of these isomers is different in ripe gh fruit. Profiling also revealed that there is an enhanced ratio of β-carotene to lycopene in the ripe gh fruit. This explains the orange colour of these fruit, and is consistent with the proposal that the β-cyclization step plays a pivotal role in regulating the partitioning of pathway intermediates. The total carotenoid content of the mutant fruit is significantly higher than normal due to an accumulation of phytoene. Phytoene accumulation, together with an up-regulation of GH transcripts during ripening, supports the notion that GH plays a central role in carotenogenesis in chromoplasts. However, GH mRNAs are also expressed in tissues and organs other than fruit. This is consistent with the possibility that GH mediates quinol oxidation from a variety of pathways that converge on plastoquinol in plastid membranes.
Scientific Publication
You may also be interested in