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Characterization of the constitutive and wound-inducible monoterpene cyclases of grand fir (Abies grandis)
Year:
1991
Authors :
Lewinsohn, Efraim
;
.
Volume :
289
Co-Authors:
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Facilitators :
From page:
267
To page:
273
(
Total pages:
7
)
Abstract:
Monoterpene cyclase activity is greatly increased in grand fir (Abies grandis) sapling stems in response to wounding and the composition of the cyclic olefin mixture generated differs from that produced constitutively as determined by radio gas-liquid chromatography. Cell-free extracts from wounded stems and from non-wounded controls were systematically compared for monoterpene cyclase activities following partial purification and separation of these enzymes by anion-exchange chromatography (Mono Q FPLC) and native PAGE. The increase in monoterpene cyclase activity following wounding represents both the apparent enhancement of constitutive cyclase activities and the appearance of novel cyclization enzymes that are absent in nonwounded controls. A pinene cyclase was shown to be the major wound-inducible enzyme directly responsible for oleoresin monoterpene formation and was tentatively identified as a 62-kDa protein by SDSPAGE. © 1991.
Note:
Related Files :
Abies grandis
enzyme induction
enzyme purification
plant
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terpene
trees
unclassified drug
wound
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More details
DOI :
10.1016/0003-9861(91)90471-T
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21741
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
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Scientific Publication
Characterization of the constitutive and wound-inducible monoterpene cyclases of grand fir (Abies grandis)
289
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Characterization of the constitutive and wound-inducible monoterpene cyclases of grand fir (Abies grandis)
Monoterpene cyclase activity is greatly increased in grand fir (Abies grandis) sapling stems in response to wounding and the composition of the cyclic olefin mixture generated differs from that produced constitutively as determined by radio gas-liquid chromatography. Cell-free extracts from wounded stems and from non-wounded controls were systematically compared for monoterpene cyclase activities following partial purification and separation of these enzymes by anion-exchange chromatography (Mono Q FPLC) and native PAGE. The increase in monoterpene cyclase activity following wounding represents both the apparent enhancement of constitutive cyclase activities and the appearance of novel cyclization enzymes that are absent in nonwounded controls. A pinene cyclase was shown to be the major wound-inducible enzyme directly responsible for oleoresin monoterpene formation and was tentatively identified as a 62-kDa protein by SDSPAGE. © 1991.
Scientific Publication
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