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Organelles contribute differentially to reactive oxygen species-related events during extended darkness
Year:
2011
Source of publication :
Plant physiology (source)
Authors :
Friedman, Haya
;
.
Rot, Ilona
;
.
Sollner, Evelyn
;
.
Volume :
156
Co-Authors:
Rosenwasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Rot, I., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Sollner, E., Heidelberg Institute for Plant Science, Heidelberg University, D-69120 Heidelberg, Germany
Meyer, A.J., Heidelberg Institute for Plant Science, Heidelberg University, D-69120 Heidelberg, Germany, Institut für Nutzpflanzenwissenschaften und Ressourcenschutz-Chemical Signalling, University of Bonn, D-53115 Bonn, Germany
Smith, Y., Genomic Data Analysis Unit, Hebrew University, Jerusalem 91120, Israel
Leviatan, N., Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Fluhr, R., Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
185
To page:
201
(
Total pages:
17
)
Abstract:
Treatment of Arabidopsis (Arabidopsis thaliana) leaves by extended darkness generates a genetically activated senescence program that culminates in cell death. The transcriptome of leaves subjected to extended darkness was found to contain a variety of reactive oxygen species (ROS)-specific signatures. The levels of transcripts constituting the transcriptome footprints of chloroplasts and cytoplasm ROS stresses decreased in leaves, as early as the second day of darkness. In contrast, an increase was detected in transcripts associated with mitochondrial and peroxisomal ROS stresses. The sequential changes in the redox state of the organelles during darkness were examined by redox-sensitive green fluorescent protein probes (roGFP) that were targeted to specific organelles. In plastids, roGFP showed a decreased level of oxidation as early as the first day of darkness, followed by a gradual increase to starting levels. However, in mitochondria, the level of oxidation of roGFP rapidly increased as early as the first day of darkness, followed by an increase in the peroxisomal level of oxidation of roGFP on the second day. No changes in the probe oxidation were observed in the cytoplasm until the third day. The increase in mitochondrial roGFP degree of oxidation was abolished by sucrose treatment, implying that oxidation is caused by energy deprivation. The dynamic redox state visualized by roGFP probes and the analysis of microarray results are consistent with a scenario in which ROS stresses emanating from the mitochondria and peroxisomes occur early during darkness at a presymptomatic stage and jointly contribute to the senescence program. © 2011 American Society of Plant Biologists.
Note:
Related Files :
antioxidants
arabidopsis
Arabidopsis thaliana
chlorophyll
Genetics
metabolism
reactive oxygen species
Show More
Related Content
More details
DOI :
10.1104/pp.110.169797
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21794
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
You may also be interested in
Scientific Publication
Organelles contribute differentially to reactive oxygen species-related events during extended darkness
156
Rosenwasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Rot, I., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Sollner, E., Heidelberg Institute for Plant Science, Heidelberg University, D-69120 Heidelberg, Germany
Meyer, A.J., Heidelberg Institute for Plant Science, Heidelberg University, D-69120 Heidelberg, Germany, Institut für Nutzpflanzenwissenschaften und Ressourcenschutz-Chemical Signalling, University of Bonn, D-53115 Bonn, Germany
Smith, Y., Genomic Data Analysis Unit, Hebrew University, Jerusalem 91120, Israel
Leviatan, N., Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Fluhr, R., Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Organelles contribute differentially to reactive oxygen species-related events during extended darkness
Treatment of Arabidopsis (Arabidopsis thaliana) leaves by extended darkness generates a genetically activated senescence program that culminates in cell death. The transcriptome of leaves subjected to extended darkness was found to contain a variety of reactive oxygen species (ROS)-specific signatures. The levels of transcripts constituting the transcriptome footprints of chloroplasts and cytoplasm ROS stresses decreased in leaves, as early as the second day of darkness. In contrast, an increase was detected in transcripts associated with mitochondrial and peroxisomal ROS stresses. The sequential changes in the redox state of the organelles during darkness were examined by redox-sensitive green fluorescent protein probes (roGFP) that were targeted to specific organelles. In plastids, roGFP showed a decreased level of oxidation as early as the first day of darkness, followed by a gradual increase to starting levels. However, in mitochondria, the level of oxidation of roGFP rapidly increased as early as the first day of darkness, followed by an increase in the peroxisomal level of oxidation of roGFP on the second day. No changes in the probe oxidation were observed in the cytoplasm until the third day. The increase in mitochondrial roGFP degree of oxidation was abolished by sucrose treatment, implying that oxidation is caused by energy deprivation. The dynamic redox state visualized by roGFP probes and the analysis of microarray results are consistent with a scenario in which ROS stresses emanating from the mitochondria and peroxisomes occur early during darkness at a presymptomatic stage and jointly contribute to the senescence program. © 2011 American Society of Plant Biologists.
Scientific Publication
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