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Effects of intramammary infusions of casein hydrolysate, ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, and lactose at drying-off on mammary gland involution
Year:
2014
Source of publication :
Journal of Dairy Science
Authors :
Silanikove, Nissim
;
.
Volume :
97
Co-Authors:
Ponchon, B., Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada
Lacasse, P., Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, J1M 0C8, Canada
Silanikove, N., Agricultural Research Organization, Volcani Center, Institute of Animal Science, PO Box 6, Bet Dagan, 50250, Israel
Ollier, S., Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, J1M 0C8, Canada
Zhao, X., Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada
Facilitators :
From page:
779
To page:
788
(
Total pages:
10
)
Abstract:
The transition from the lactation to the dry period in dairy cows is a period of high risk for acquiring new intramammary infections. This risk is reduced when involution of mammary glands is completed. Consequently, strategies that accelerate the involution process after drying-off could reduce the incidence of mastitis. The objective of this study was to assess the effect of 3 different treatments on mammary gland involution. Each quarter of 8 Holstein cows in late lactation was randomly assigned at drying-off to an intramammary infusion of casein hydrolysate (CNH; 70mg), ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA; 5.7g), lactose (5.1g), or saline 0.9% (control) solutions. Milk samples were collected on the last 2 d before and 1, 3, 5, 7, 10, and 14 d after the last milking for determining concentrations of mammary gland involution markers. Lactoferrin, somatic cell counts (SCC), BSA, and Na+ concentrations, as well as matrix metalloproteinase-2 and matrix metalloproteinase-9 activities gradually increased in mammary secretions during the first 2 wk following the last milking, whereas milk citrate and K+ concentrations decreased. As involution advanced, the Na+:K+ ratio increased, whereas the citrate:lactoferrin ratio decreased. Compared with mammary secretions from control quarters, mammary secretions of quarters infused with CNH had higher SCC on d 1, 3, 5, and 7, and greater BSA concentrations on d 1, 3, and 5. Similarly, the CNH treatment induced a faster increase in lactoferrin concentrations, which were greater than in milk from control quarters on d 3, 5, and 7 after drying-off. Milk citrate concentrations were unaffected by CNH but the citrate:lactoferrin ratio was lower in CNH-treated quarters on d 3 and 5 than in control quarters. Moreover, CNH treatment hastened the increase in Na+ concentration and in the Na+:K+ ratio on d 1. Infusion of CNH also led to an increase in proteolytic activities, with greater matrix metalloproteinase 9 activities on d 1 and 3. The EGTA infusion increased SCC above that of control quarters on d 1 and 3 but it had no effect on the other parameters. Lactose infusion had no effect on any of the involution markers. In this study, intramammary infusions of CNH were the most efficient treatment to accelerate mammary gland involution, suggesting a potential role of CNH as a local milk secretion inhibitor during milk stasis. © 2014 American Dairy Science Association.
Note:
Related Files :
Animal
Animals
calcium chelating agent
casein hydrolysate
cattle
chemistry
Female
milk
veterinary
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Related Content
More details
DOI :
10.3168/jds.2013-7062
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21796
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
You may also be interested in
Scientific Publication
Effects of intramammary infusions of casein hydrolysate, ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, and lactose at drying-off on mammary gland involution
97
Ponchon, B., Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada
Lacasse, P., Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, J1M 0C8, Canada
Silanikove, N., Agricultural Research Organization, Volcani Center, Institute of Animal Science, PO Box 6, Bet Dagan, 50250, Israel
Ollier, S., Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, J1M 0C8, Canada
Zhao, X., Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada
Effects of intramammary infusions of casein hydrolysate, ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, and lactose at drying-off on mammary gland involution
The transition from the lactation to the dry period in dairy cows is a period of high risk for acquiring new intramammary infections. This risk is reduced when involution of mammary glands is completed. Consequently, strategies that accelerate the involution process after drying-off could reduce the incidence of mastitis. The objective of this study was to assess the effect of 3 different treatments on mammary gland involution. Each quarter of 8 Holstein cows in late lactation was randomly assigned at drying-off to an intramammary infusion of casein hydrolysate (CNH; 70mg), ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA; 5.7g), lactose (5.1g), or saline 0.9% (control) solutions. Milk samples were collected on the last 2 d before and 1, 3, 5, 7, 10, and 14 d after the last milking for determining concentrations of mammary gland involution markers. Lactoferrin, somatic cell counts (SCC), BSA, and Na+ concentrations, as well as matrix metalloproteinase-2 and matrix metalloproteinase-9 activities gradually increased in mammary secretions during the first 2 wk following the last milking, whereas milk citrate and K+ concentrations decreased. As involution advanced, the Na+:K+ ratio increased, whereas the citrate:lactoferrin ratio decreased. Compared with mammary secretions from control quarters, mammary secretions of quarters infused with CNH had higher SCC on d 1, 3, 5, and 7, and greater BSA concentrations on d 1, 3, and 5. Similarly, the CNH treatment induced a faster increase in lactoferrin concentrations, which were greater than in milk from control quarters on d 3, 5, and 7 after drying-off. Milk citrate concentrations were unaffected by CNH but the citrate:lactoferrin ratio was lower in CNH-treated quarters on d 3 and 5 than in control quarters. Moreover, CNH treatment hastened the increase in Na+ concentration and in the Na+:K+ ratio on d 1. Infusion of CNH also led to an increase in proteolytic activities, with greater matrix metalloproteinase 9 activities on d 1 and 3. The EGTA infusion increased SCC above that of control quarters on d 1 and 3 but it had no effect on the other parameters. Lactose infusion had no effect on any of the involution markers. In this study, intramammary infusions of CNH were the most efficient treatment to accelerate mammary gland involution, suggesting a potential role of CNH as a local milk secretion inhibitor during milk stasis. © 2014 American Dairy Science Association.
Scientific Publication
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