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A defect in nir1, a nirA-like transcription factor, confers morphological abnormalities and loss of pathogenicity in Colletotrichum acutatum
Year:
2006
Source of publication :
Molecular Plant Pathology
Authors :
Freeman, Stanley
;
.
Horowitz, Sigal Brown
;
.
Zveibil, Aida
;
.
Volume :
7
Co-Authors:
Horowitz, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
341
To page:
354
(
Total pages:
14
)
Abstract:
A non-pathogenic mutant of Colletotrichum acutatum, designated Ca5, exhibited epiphytic hyphal growth and did not cause lesions on strawberry plants but grew necrotrophically when inoculated directly onto wounded stolons. In the absence of an external nitrogen source, the mutant exhibited extended germ-tube growth prior to appressorium formation. The deduced product of the impaired gene (nir1) is similar to NirA, an Aspergillus nidulans transcriptional regulator of nitrogen metabolism. Inoculation of leaves with wild-type or Ca5 conidia in the presence of a preferred nitrogen source resulted in massive epiphytic hyphal production, appressorium formation and rapid symptom development. Expression of C. acutatum wild-type nitrate reductase (nit1) and glutamine synthetase (gln1) was induced by nitrate but only nit1 expression was repressed in a rich medium. nit1 transcription increased during the appressorium-production stage, indicating that nitrogen starvation constitutes a cue for the regulation of appressorium development. The presence of nit1 transcript during various phases of infection is indicative of partial nitrogen starvation in planta. cAMP-dependent protein kinase A (PKA) was determined to be a negative regulator of immediate post-germination appressoria formation in the wild-type. As inhibition of PKA activity in the nir1 mutant did not affect appressoria formation, we suggest that NIR1 acts either in parallel or downstream of the PKA pathway. Our results show that nir1 is a pathogenicity determinant and a regulator of pre-infection development under nitrogen-starvation conditions and that nitrogen availability is a significant factor in the pre-penetration phase. © 2006 Blackwell Publishing Ltd.
Note:
Related Files :
Colletotrichum
Emericella nidulans
Fragaria x ananassa
Glomerella acutata
Show More
Related Content
More details
DOI :
10.1111/j.1364-3703.2006.00341.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21821
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Scientific Publication
A defect in nir1, a nirA-like transcription factor, confers morphological abnormalities and loss of pathogenicity in Colletotrichum acutatum
7
Horowitz, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
A defect in nir1, a nirA-like transcription factor, confers morphological abnormalities and loss of pathogenicity in Colletotrichum acutatum
A non-pathogenic mutant of Colletotrichum acutatum, designated Ca5, exhibited epiphytic hyphal growth and did not cause lesions on strawberry plants but grew necrotrophically when inoculated directly onto wounded stolons. In the absence of an external nitrogen source, the mutant exhibited extended germ-tube growth prior to appressorium formation. The deduced product of the impaired gene (nir1) is similar to NirA, an Aspergillus nidulans transcriptional regulator of nitrogen metabolism. Inoculation of leaves with wild-type or Ca5 conidia in the presence of a preferred nitrogen source resulted in massive epiphytic hyphal production, appressorium formation and rapid symptom development. Expression of C. acutatum wild-type nitrate reductase (nit1) and glutamine synthetase (gln1) was induced by nitrate but only nit1 expression was repressed in a rich medium. nit1 transcription increased during the appressorium-production stage, indicating that nitrogen starvation constitutes a cue for the regulation of appressorium development. The presence of nit1 transcript during various phases of infection is indicative of partial nitrogen starvation in planta. cAMP-dependent protein kinase A (PKA) was determined to be a negative regulator of immediate post-germination appressoria formation in the wild-type. As inhibition of PKA activity in the nir1 mutant did not affect appressoria formation, we suggest that NIR1 acts either in parallel or downstream of the PKA pathway. Our results show that nir1 is a pathogenicity determinant and a regulator of pre-infection development under nitrogen-starvation conditions and that nitrogen availability is a significant factor in the pre-penetration phase. © 2006 Blackwell Publishing Ltd.
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