נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Synthesis and secretion of human serum albumin by mammary gland explants of virgin and lactating transgenic mice
Year:
1993
Source of publication :
Transgenic Research
Authors :
Barash, Itamar
;
.
Baruch, Ariela
;
.
Faerman, Alexander
;
.
Shani, Moshe
;
.
Volume :
2
Co-Authors:
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Baruch, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Nathan, M., Rhone-Poulenc Rorer Central Research, Collegeville, 19426, PA, United States
Hurwitz, D.R., Rhone-Poulenc Rorer Central Research, Collegeville, 19426, PA, United States
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
266
To page:
276
(
Total pages:
11
)
Abstract:
Transgenic mice were produced, carrying hybrid genes comprised of the ovine β-lactoglobulin (BLG) milk protein gene promoter and human serum albumin (HSA) coding sequences. In situ hybridization revealed high levels of BLG/HSA hybrid mRNA, confined to the epithelial cells of the lactating mammary gland with a several hundred fold lower concentration in virgin mammary glands. During the first 24 h in culture, exceptionally high levels of HSA were secreted from explants of virgin mice, independent of hormonal control. HSA secretion was reduced considerably during subsequent days in culture and became dependent on the presence of insulin, hydrocortisone and prolactin. This temporal and hormonal pattern of regulation of HSA was different than that found for the secretion of caseins. In contrast to the vast difference in the mRNA content, the amount of HSA secreted from explants derived from lactating mice during the first 24 h in culture was only 2-to 5-fold higher than that found with explants from virgin transgenic mice, suggesting post-transcriptional control of HSA synthesis. The high-level synthesis and secretion of HSA in mammary explants of lactating mice was also dependent on the presence of insulin, hydrocortisone and prolactin. This study confirms previous suggestion that mammary explants from virgin transgenics may serve as a powerful tool for screening the potential of transgenic animals to secrete foreign proteins in their milk. © 1993 Chapman & Hall.
Note:
Related Files :
Animal
biosynthesis
Female
Genetics
lactation
metabolism
mice
Serum Albumin
sheep
tissue culture
Show More
Related Content
More details
DOI :
10.1007/BF01968839
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21836
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
You may also be interested in
Scientific Publication
Synthesis and secretion of human serum albumin by mammary gland explants of virgin and lactating transgenic mice
2
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Baruch, A., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Nathan, M., Rhone-Poulenc Rorer Central Research, Collegeville, 19426, PA, United States
Hurwitz, D.R., Rhone-Poulenc Rorer Central Research, Collegeville, 19426, PA, United States
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Synthesis and secretion of human serum albumin by mammary gland explants of virgin and lactating transgenic mice
Transgenic mice were produced, carrying hybrid genes comprised of the ovine β-lactoglobulin (BLG) milk protein gene promoter and human serum albumin (HSA) coding sequences. In situ hybridization revealed high levels of BLG/HSA hybrid mRNA, confined to the epithelial cells of the lactating mammary gland with a several hundred fold lower concentration in virgin mammary glands. During the first 24 h in culture, exceptionally high levels of HSA were secreted from explants of virgin mice, independent of hormonal control. HSA secretion was reduced considerably during subsequent days in culture and became dependent on the presence of insulin, hydrocortisone and prolactin. This temporal and hormonal pattern of regulation of HSA was different than that found for the secretion of caseins. In contrast to the vast difference in the mRNA content, the amount of HSA secreted from explants derived from lactating mice during the first 24 h in culture was only 2-to 5-fold higher than that found with explants from virgin transgenic mice, suggesting post-transcriptional control of HSA synthesis. The high-level synthesis and secretion of HSA in mammary explants of lactating mice was also dependent on the presence of insulin, hydrocortisone and prolactin. This study confirms previous suggestion that mammary explants from virgin transgenics may serve as a powerful tool for screening the potential of transgenic animals to secrete foreign proteins in their milk. © 1993 Chapman & Hall.
Scientific Publication
You may also be interested in