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Characterization of the Erwinia amylovora population in Israel
Year:
1997
Source of publication :
Phytoparasitica
Authors :
David, Israel
;
.
Dror, Orit
;
.
Kleitman, Frida
;
.
Zutra, Dan
;
.
Volume :
26
Co-Authors:
Manulis, S., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Kleitman, F., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Dror, O., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
David, I., Dept. of Fruit Trees, ARO, Volcani Center, Bet Dagan 50250, Israel
Zutra, D., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
39
To page:
46
(
Total pages:
8
)
Abstract:
A collection of 205 strains of Erwinia amylovora isolated in Israel over a period of 12 years has been established. The strains were isolated from different varieties of pear, apple, loquat and quince grown in Israel, and collected from different locations in the country. They were characterized in respect to degree of virulence on several hosts and serological and molecular characters. Pathogenicity tests carried out on flowering branches of pear and apple, shoots of pears, and on trees of pear and loquat grown in containers outdoors, revealed no significant differences in the severity of blossom blight or shoot blight among the various strains. ELISA and immunofluorescence assays revealed no serotypic groups among the Israeli strains. Genomic diversity was studied by random amplified polymorphic DNA (RAPD) analysis using 24 arbitrary 10-base primers. All the strains examined (45 Israeli and 11 from Egypt, Cyprus and Greece) produced the same RAPD patterns with each of the primers used. Amplification patterns were indistinguishable from those produced by strains isolated from the neighboring countries. Results presented in this study suggest that the population of E. amylovora in Israel is homogenous.
Note:
Related Files :
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fire blight
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21838
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Scientific Publication
Characterization of the Erwinia amylovora population in Israel
26
Manulis, S., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Kleitman, F., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Dror, O., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
David, I., Dept. of Fruit Trees, ARO, Volcani Center, Bet Dagan 50250, Israel
Zutra, D., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Characterization of the Erwinia amylovora population in Israel
A collection of 205 strains of Erwinia amylovora isolated in Israel over a period of 12 years has been established. The strains were isolated from different varieties of pear, apple, loquat and quince grown in Israel, and collected from different locations in the country. They were characterized in respect to degree of virulence on several hosts and serological and molecular characters. Pathogenicity tests carried out on flowering branches of pear and apple, shoots of pears, and on trees of pear and loquat grown in containers outdoors, revealed no significant differences in the severity of blossom blight or shoot blight among the various strains. ELISA and immunofluorescence assays revealed no serotypic groups among the Israeli strains. Genomic diversity was studied by random amplified polymorphic DNA (RAPD) analysis using 24 arbitrary 10-base primers. All the strains examined (45 Israeli and 11 from Egypt, Cyprus and Greece) produced the same RAPD patterns with each of the primers used. Amplification patterns were indistinguishable from those produced by strains isolated from the neighboring countries. Results presented in this study suggest that the population of E. amylovora in Israel is homogenous.
Scientific Publication
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