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Baculovirus-mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins
Year:
1998
Source of publication :
FEBS Letters
Authors :
Chejanovsky, Nor
;
.
Volume :
422
Co-Authors:
Gershburg, E., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel, Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Stockholm, D., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel
Froy, O., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Rashi, S., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Gurevitz, M., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel
Facilitators :
From page:
132
To page:
136
(
Total pages:
5
)
Abstract:
The insecticidal efficacy towards Helicoverpa armigera lepidopteran larvae of recombinant Autographa californica M nucleopolyhedroviruses, expressing depressant and excitatory scorpion anti-insect selective toxins, was investigated. The ET 50 (effective paralysis time 50%) values obtained with the recombinant viruses expressing the depressant toxin, LqhIT2, and the excitatory toxin, LqhIT1, were 59 h and 66 h, respectively, whereas the ET 50 value of the wild-type virus was longer, 87 h post infection. The insecticidal effects obtained when using two distinct temporally regulated viral promoters revealed advantage for the very late p10 promoter over the p35 early promoter. The higher insecticidity of the virus expressing the depressant toxin compared to the excitatory toxin suggests that pharmacokinetic factors and/or promoter efficiency may play a role during infection of insect pest larvae by recombinant baculoviruses.
Note:
Related Files :
animal cell
Animals
Baculoviridae
gene expression
insect cell
insecticides
insects
Lepidoptera
virus vector
Show More
Related Content
More details
DOI :
10.1016/S0014-5793(97)01614-1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21847
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Scientific Publication
Baculovirus-mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins
422
Gershburg, E., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel, Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Stockholm, D., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel
Froy, O., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Rashi, S., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Gurevitz, M., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan 50250, Israel
Baculovirus-mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins
The insecticidal efficacy towards Helicoverpa armigera lepidopteran larvae of recombinant Autographa californica M nucleopolyhedroviruses, expressing depressant and excitatory scorpion anti-insect selective toxins, was investigated. The ET 50 (effective paralysis time 50%) values obtained with the recombinant viruses expressing the depressant toxin, LqhIT2, and the excitatory toxin, LqhIT1, were 59 h and 66 h, respectively, whereas the ET 50 value of the wild-type virus was longer, 87 h post infection. The insecticidal effects obtained when using two distinct temporally regulated viral promoters revealed advantage for the very late p10 promoter over the p35 early promoter. The higher insecticidity of the virus expressing the depressant toxin compared to the excitatory toxin suggests that pharmacokinetic factors and/or promoter efficiency may play a role during infection of insect pest larvae by recombinant baculoviruses.
Scientific Publication
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